Protein Information

General Information
MoonProt ID112
First appeared in release1.0
Name(s)Intron-encoded DNA endonuclease I-AniI COB intron protein mRNA maturase bI1 Gene Name: I-AniI
UniProt IDP03880 (ANI1_EMEND), Reviewed
GO termsGO:0006314 intron homing GO:0006397 mRNA processing GO:0008380 RNA splicing GO:0090305 nucleic acid phosphodiester bond hydrolysis GO:0003677 DNA binding GO:0004518 nuclease activity GO:0004519 endonuclease activity GO:0016787 hydrolase activity GO:0005739 mitochondrion GO:0004519 endonuclease activity GO:0006314 intron homing
Organisms for which functions have been demonstratedEmericella nidulans (Aspergillus nidulans)
Sequence length488
FASTA sequence>sp|P03880|ANI1_EMEND Intron-encoded DNA endonuclease I-AniI OS=Emericella nidulans GN=I-AniI PE=1 SV=3 MRILKSHPLLKIVNSYIIDSPQPANLSYLWNFGSLLALCLGIQIVTGVTLAMHYTPSVSEAFNSVEHIMRDVNNGWLVRYLHSNTASAFFFLVYLHIGRGLYYGSYKTPRTLTWAIGTVILIVMMATAFLGYVLPYGQMSLWGATVITNLMSAIPWIGQDIVEFIWGGLYTDEPQCGDVLLKILLNAGKSPILGFAYDLFFIIVLLIGVKIAMTRGKSAGVRSLHTSEASQRLHAGDLTYAYLVGLFEGDGYFSITKKGKYLTYELGIELSIKDVQLIYKIKKILGIGIVSFRKINEIEMVALRIRDKNHLKSFILPIFEKYPMFSNKQYDYLRFRNALLSGIISLEDLPDYTRSDEPLNSIESIINTSYFSAWLVGFIEAEGCFSVYKLNKDDDYLIASFDIAQRDGDILISAIRKYLSFTTKVYLDKTNCSKLKVTSVRSVENIIKFLQNAPVKLLGNKKLQYLLWLKQLRKISRYSEKIKIPSNY
Structure Information
PDB ID2QOJ 3EH8
Quaternary structure
Function 1
Function descriptionmRNA maturase required for splicing of the intron in the cytochrome b (cobA) gene, which contains the coding sequence for the maturase. stimulates the intrinsic ribozyme activity of the intron by binding to and stabilizing the three-dimensional structure of the RNA.
References for functionBolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL. Structural and biochemical analyses of DNA and RNA binding a bifunctional homing endonuclease and group I intron splicing factor. Genes Dev. 2003 Dec 1;17(23):2875-88. PMID: 14633971 Ho Y., Kim S.J., Waring R.B. A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. (1997). Proc. Natl. Acad. Sci. 94:8994-8999. PMID: 9256423
E.C. number3.1.26 or 3.1.27, 6.5.1.3?
Location of functional site(s)
Cellular location of functionmitochondrion
Comments
Function 2
Function descriptionhoming endonuclease introduces a double-strand break at a specific location in the cobA gene and helps in the insertion of an intron containing its own coding sequence (group I intron)
References for functionBolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL. Structural and biochemical analyses of DNA and RNA binding a bifunctional homing endonuclease and group I intron splicing factor. Genes Dev. 2003 Dec 1;17(23):2875-88. PMID: 14633971 Ho Y., Kim S.J., Waring R.B. A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. (1997). Proc. Natl. Acad. Sci. 94:8994-8999. PMID: 9256423
E.C. number3.1.21 or 3.1.22
Location of functional site(s)
Cellular location of functionmitochondrion
Comments