Protein Information

General Information
MoonProt ID113
First appeared in release1.0
Name(s)Arg5,6p N-Acetylglutamate kinase (Arg5)and N-acetylglutamyl-phosphate reductase (Arg6) **Note this is considered a moonlighting protein not because of the two enzyme activities but because it is both an enzyme and a transcription factor*** Protein ARG5,6, mitochondrial N-acetyl-glutamate semialdehyde dehydrogenase NAGSA dehydrogenase N-acetyl-L-glutamate 5-phosphotransferase NAG kinase AGK Gene Name: ARG5,6
UniProt IDQ01217 (ARG56_YEAST), Reviewed
GO termsGO:0006355 regulation of transcription, DNA-templated GO:0006526 arginine biosynthetic process GO:0006592 ornithine biosynthetic process GO:0008152 metabolic process GO:0008652 cellular amino acid biosynthetic process GO:0016310 phosphorylation GO:0055114 oxidation-reduction process GO:0000166 nucleotide binding GO:0003824 catalytic activity GO:0003942 N-acetyl-gamma-glutamyl-phosphate reductase activity GO:0003991 acetylglutamate kinase activity GO:0005524 ATP binding GO:0016301 kinase activity GO:0016491 oxidoreductase activity GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0016740 transferase activity GO:0046983 protein dimerization activity GO:0051287 NAD binding GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0003677 DNA binding GO:0006355 regulation of transcription, DNA-templated
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length863
FASTA sequence>sp|Q01217|ARG56_YEAST Protein ARG5,6, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ARG5,6 PE=1 SV=1 MPSASLLVSTKRLNASKFQKFVSSLNKSTIAGFASVPLRAPPSVAFTRKKVGYSKRYVSSTNGFSATRSTVIQLLNNISTKREVEQYLKYFTSVSQQQFAVIKVGGAIISDNLHELASCLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRITDEHTMAVVRKCFLEQNLKLVTALEQLGVRARPITSGVFTADYLDKDKYKLVGNIKSVTKEPIEASIKAGALPILTSLAETASGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEKISMINLDEEYDDLMKQSWVKYGTKLKIREIKELLDYLPRSSSVAIINVQDLQKELFTDSGAGTMIRRGYKLVKRSSIGEFPSADALRKALQRDAGISSGKESVASYLRYLENSDFVSYADEPLEAVAIVKKDTNVPTLDKFVCSDAAWLNNVTDNVFNVLRRDFPALQWVVSENDANIAWHFDKSQGSYLKGGKVLFWYGIDDINTISELVENFVKSCDTASTLNSSASSGVFANKKSARSYSTRSTPRPEGVNTNPGRVALIGARGYTGKNLVSLINGHPYLEVAHVSSRELKGQKLQDYTKSEIIYESLQIQDIRKLEEQNAVDFWVMALPNKVCEPFVETIQSVHGKSKIIDLSADHRFVSESDWAYGLPELNDRAKIANAAKIANPGCYATGSQLTISPLTKYINGLPTVFGVSGYSGAGTKPSPKNDPKFLNNNLIPYALSDHIHEREISARIGHNVAFMPHVGQWFQGISLTVSIPIKKGSLSIDEIRKLYRNFYEDEKLVHVIDDIPLVKDIEGTHGVVIGGFKLNDAEDRVVVCATIDNLLKGAATQCLQNINLAMGYGEYAGIPENKIIGV
Structure Information
PDB ID3ZZI 3ZZF
Quaternary structure
Function 1
Function descriptionenzyme in the arginine biosynthetic pathway N-acetyl-gamma phosphate reductase and acetyl glutamate kinase
References for functionDetermination of amino acid sequences involved in the processing of the ARG5/ARG6 precursor in Saccharomyces cerevisiae. Boonchird,C., Messenguy,F.,and Dubois,E. (1991) Eur. J. Biochem. 199, 325-335.PMID: 1649049 A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis A. Abadjieva, K. Pauwels, P. Hilven, and M. Crabeel.J Biol Chem. 2001 Nov 16;276(46):42869-80. Epub 2001 Sep 11. PMID: 11553611
E.C. numberEC 2.7.2.8 and EC 1.2.1.38
Location of functional site(s)Active site:675
Cellular location of functionmitochondria
Comments
Function 2
Function descriptionArg5 binds to mitochondrial and nuclear DNA in vivo and regulates gene expression, regulator of transcription for several genes
References for functionRegulation of gene expression by a metabolic enzyme. Hall DA, Zhu H, Zhu X, Royce T, Gerstein M, Snyder M. Science. 2004 Oct 15; 306(5695): 482-4. PMID: 15486299
E.C. numberN/A
Location of functional site(s)
Cellular location of functionnucleus and mitochondria
Comments