Protein Information

General Information
MoonProt ID130
First appeared in release1.0
Name(s)Phosphoglycerate kinase 1 Cell migration-inducing gene 10 protein Primer recognition protein 2 Plasmin Reductase PGKA PRP 2 Gene Name: PGK1
UniProt IDP00558 (PGK1_HUMAN) Reviewed
GO termsGO:0005975 carbohydrate metabolic process GO:0006006 glucose metabolic process GO:0006094 gluconeogenesis GO:0006096 glycolysis GO:0016310 phosphorylation GO:0030855 epithelial cell differentiation GO:0044281 small molecule metabolic process GO:0000166 nucleotide binding GO:0004618 phosphoglycerate kinase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0005737 cytoplasm GO:0005829 cytosol GO:0070062 extracellular vesicular exosome GO:0055114 oxidation-reduction process
Organisms for which functions have been demonstratedHomo sapiens (Human)
Sequence length417
FASTA sequence>gi|4505763|ref|NP_000282.1| phosphoglycerate kinase 1 [Homo sapiens] MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
Structure Information
PDB ID2WZB 2WZC 2WZD 2X13 2X14 2X15 2XE6 2XE7 2XE8 2Y3I 2YBE 2ZGV 3C39 3C3A 3C3B 3C3C
Quaternary structure
Function 1
Function descriptionPhosphoglycerate Kinase, enzyme ADP + 1,3-bisphosphoglycerate => ATP + 3-phospho-D-glycerate Carbohydrate degradation, glycolysis
References for functionMichelson AM, Markham AF, Orkin SH. Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Natl Acad Sci U S A. 1983 Jan. PMID: 6188151
E.C. number2.7.2.3
Location of functional site(s)Binding site:313,39,171,123,344,220 Nucleotide binding site:373-376
Cellular location of functioncytoplasm
CommentsSubtrate binding site of 1,3-bisphosphoglycerate (bPG): Asp23, Asn25, Arg38, His62, Arg65, Arg122, Arg170. Subtrate Binding site of ADP: Ala214, Lys215, Lys219, Gly312, Asn336, Glu343, Asp374, Thr375. Reference: Transition State Analogue Structures of Human Phosphoglycerate Kinase Establish the Importance of Charge Balance in Catalysis Matthew J. Cliff, Matthew W. Bowler, Andrea Varga, James P. Marston, Judit Szab, Andrea M. Hounslow, Nicola J. Baxter, G. Michael Blackburn, Mria Vas, and Jonathan P. Waltho. Journal of the American Chemical Society 2010 132 (18), 6507-6516 PubMed ID (20397725) DOI (10.1021/ja100974t)
Function 2
Function descriptiondisulphide reductase, enzyme Inhibits angiogenesis through a disulfide reductase activity that activates plasminogen autoproteolytic activity, producing angiostatin. One or more of the PGK cysteine residues may have a role in plasmin reduction.
References for functionLay AJ, Jiang XM, Kisker O, Flynn E, Underwood A, Condron R, Hogg PJ. Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase. Nature. 2000 Dec 14. PMID: 11130727
E.C. numberN/A
Location of functional site(s)
Cellular location of functionExtracellular. Secreted Protein.
Comments