Protein Information

General Information
MoonProt ID132
First appeared in release1.0
Name(s)Mismatch repair endonuclease PMS2 Mismatch repair endonuclease PMS2 Alternative name(s): DNA mismatch repair protein PMS2 PMS1 protein homolog 2 Gene names Name: Pms2
UniProt IDP54279 (PMS2_MOUSE) Reviewed
GO termsGO:0006200 ATP catabolic process GO:0006281 DNA repair GO:0006298 mismatch repair GO:0006974 cellular response to DNA damage stimulus GO:0007126 meiotic nuclear division GO:0007131 reciprocal meiotic recombination GO:0016446 somatic hypermutation of immunoglobulin genes GO:0016447 somatic recombination of immunoglobulin gene segments GO:0090305 nucleic acid phosphodiester bond hydrolysis GO:0003697 single-stranded DNA binding GO:0004518 nuclease activity GO:0004519 endonuclease activity GO:0005524 ATP binding GO:0016787 hydrolase activity GO:0016887 ATPase activity GO:0030983 mismatched DNA binding GO:0032407 MutSalpha complex binding GO:0005634 nucleus GO:0032389 MutLalpha complex GO:0016446 somatic hypermutation of immunoglobulin genes
Organisms for which functions have been demonstratedMus musculus
Sequence length859
FASTA sequence>sp|P54279|PMS2_MOUSE Mismatch repair endonuclease PMS2 OS=Mus musculus GN=Pms2 PE=2 SV=1 MEQTEGVSTECAKAIKPIDGKSVHQICSGQVILSLSTAVKELIENSVDAGATTIDLRLKDYGVDLIEVSDNGCGVEEENFEGLALKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHGSASVGTRLVFDHNGKITQKTPYPRPKGTTVSVQHLFYTLPVRYKEFQRNIKKEYSKMVQVLQAYCIISAGVRVSCTNQLGQGKRHAVVCTSGTSGMKENIGSVFGQKQLQSLIPFVQLPPSDAVCEEYGLSTSGRHKTFSTFRASFHSARTAPGGVQQTGSFSSSIRGPVTQQRSLSLSMRFYHMYNRHQYPFVVLNVSVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDANKLNVNQQPLLDVEGNLVKLHTAELEKPVPGKQDNSPSLKSTADEKRVASISRLREAFSLHPTKEIKSRGPETAELTRSFPSEKRGVLSSYPSDVISYRGLRGSQDKLVSPTDSPGDCMDREKIEKDSGLSSTSAGSEEEFSTPEVASSFSSDYNVSSLEDRPSQETINCGDLDCRPPGTGQSLKPEDHGYQCKALPLARLSPTNAKRFKTEERPSNVNISQRLPGPQSTSAAEVDVAIKMNKRIVLLEFSLSSLAKRMKQLQHLKAQNKHELSYRKFRAKICPGENQAAEDELRKEISKSMFAEMEILGQFNLGFIVTKLKEDLFLVDQHAADEKYNFEMLQQHTVLQAQRLITPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDEDAPVTERAKLISLPTSKNWTFGPQDIDELIFMLSDSPGVMCRPSRVRQMFASRACRKSVMIGTALNASEMKKLITHMGEMDHPWNCPHGRPTMRHVANLDVISQN
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionPMS2 mismatch repair enzyme introduces single-strand breaks near the mismatch
References for functionS.M. Baker, C.E. Bronner, L. Zhang, et al. (1995) Male Mice Defective in the DNA Mismatch Repair Gene PMS2 Exhibit Abnormal Chromosome Synapsis in Meiosis. Cell. 82, 309-319.
E.C. number3.1.- -
Location of functional site(s)
Cellular location of functionnucleus
CommentsProtein-DNA binding: Pro14, Pro15, Leu16, Gly37, Asp38, Phe39, Glu41, Leu56, Val57, Ser63, Lys64, Met70, Gly72, Pro74, Arg76, Gly106, Val108, Arg110, Lys439, Asn443, Val445, Thr453, Arg454, Gln468, Leu470, Lys471, Arg473, Arg475. G. Obmolova, C. Ban, P. Hsieh and W. Yang. (2000) Crystal structure of mismatch repair protein MutS and its complex with a substrate DNA. Nature. 407, 703-710. Nucleotide binding: Glu41, Asn45, Asp70, Thr155. A. Guarne, M.S. Junop and W. Yang. (2001) Structure and function of the N-terminal 40kDa fragment of human PMS2: a monomeric GHL ATPase. The EMBO J. 20, 5521-5531. DNA binding: Tyr868, Gly891. S. Gupta, M. Gellert and W. Yang. (2012) Mechanism of mismatch recognition revealed by human MutS? bound to unpaired DNA loops. Nature. 19, 72-79. DNA binding(from E.Coli, with 96% Query Cover and 30% Max Ident compared to human MSH3): Met33, Asp35, Phe36, Glu38. M.H. Lamers, A. Perrakis, J.H. Enzlin, et al. (2000) Nature. 407, 711-717.
Function 2
Function descriptionHypermutation of antibody variable chains
References for functionD.B. Winter, Q.H. Phung, A Umar, et al. (1998) Altered spectra of hypermutation in antibodies from mice deficient for the DNA mismatch repair protein PMS2. Proc. Natl. Acad. Sci. 95, 6953-6958. M. Wiesendanger, B. Kneitz, W. Edelmann, and M.D. Scharff. (2000) Somatic Hypermutation in MutS Homologue(MSH)3-, MSH6-, and MSH3/MSH6-deficient Mice Reveals a Role for the MSH2-MSH6 Heterodimer in Modulating the Base Sustitution Pattern. J. Exp. Med. 191, 579-584. Z. Li, S.J. Scherer, D. Ronai, et al. (2004) Examination of Msh6- and Msh3- deficient Mice in Class Switching Reveals Overlapping and Distinct Roles of MutS Homologues in Antibody Diversification. J. Exp. Med. 200, 47-59. C.E. Schrader, W. Edelmann, R. Kucherlapati, J. Stavnezer. (1999) Reduced Isotype Switching in Splenic B Cells from Mice Deficient in Mismatch Repair Enzymes. J. Exp. Med. 190, 323-330.
E.C. number
Location of functional site(s)
Cellular location of functionnucleus
Comments