Protein Information

General Information
MoonProt ID137
First appeared in release1.0
Name(s)General control protein GCN4 AAS3 ARG9 Amino acid biosynthesis regulatory protein Gene Name: GCN4
UniProt IDP03069 (GCN4_YEAST) Reviewed
GO termsGO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0008652 cellular amino acid biosynthetic process GO:0010691 negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels GO:0045899 positive regulation of RNA polymerase II transcriptional preinitiation complex assembly GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter GO:0001084 TFIID-class binding transcription factor activity GO:0001135 RNA polymerase II transcription factor recruiting transcription factor activity GO:0001190 RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription GO:0001191 RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription GO:0003677 DNA binding GO:0003682 chromatin binding GO:0003700 sequence-specific DNA binding transcription factor activity GO:0005515 protein binding GO:0042802 identical protein binding GO:0043565 sequence-specific DNA binding GO:0005634 nucleus GO:0004540 ribonuclease activity
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length281
FASTA sequence>sp|P03069|GCN4_YEAST General control protein GCN4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCN4 PE=1 SV=1 MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER
Structure Information
PDB ID1YSA 1ZTA 2ZTA 4DMD 4DME 3GJP
Quaternary structure
Function 1
Function descriptiontranscription factor; transcription activator
References for functionIA Hope and K Struhl. (1986) Functional Dissection of a Eukaryotic Transcriptional Activator Protein, GCN4 of yeast. Cell. 46, 885-894.
E.C. numberN/A
Location of functional site(s)
Cellular location of functionnucleus
CommentsRNA Binding: Arg3, Lys5, Glu8, Lys10, Glu12, Ser16. Y Nikolaev and K Pervushin. (2012) Structural basis of RNA binding by leucine zipper GCN4. Prot Sci. 21, 667-676. Dimer forming: Asn16. L Gonzalez, D Woolfson, and T Alber. (1996) Buried polar residues and structural specificity in the GCN4 leucine zipper. Nature Struct. Biol. 3, 1011-1018. DNA Binding: Asn235, Ala238, Ala239, Ser242, Arg243. TE Ellenberger, CJ Brandi, K Struhl, et al. (1992) The GCN4 Basic Region Leucine Zipper Binds DNA as a dimer of Uninterrupted ? Helices: Crystal Structure of the Protein-DNA Complex. Cell. 71, 1223-1237. DNA Binding: Arg249. M Okagami, M Uero, K Makino, et al. (1995) Sequence-Specific DNA Binding by Covalently Constrained Peptide Dimers of the Basic Leucine Zipper Protein GCN4. Bioorg & Med Chem. 3, 777-784. (Planys and animals leucine zipper)dimer forming and DNA binding: Leu5, Val9, Leu12, Asn16, Leu19, Val23, Leu26, Val30. T Alber. (1992) Structure of the leucine zipper. Curr Opin in Gene and Devel. 2, 205-210.
Function 2
Function descriptionribonuclease
References for functionY Nikolaev, C Deillon, S RK Hoffmann, et al. (2010) The Leucine Zipper Domains of the Transcription Factors GCN4 and c-Jun Have Ribonuclease Activity. Plos One. 5, 1-12. PMID: 20505831
E.C. number3.1.27.5
Location of functional site(s)
Cellular location of functioncytoplasm
CommentsRibonuclease active sites: Glu12, Glu13, Glu58, Ser16, His20, His92 Y Nikolaev, C Deillon, S RK Hoffmann, et al. (2010) The Leucine Zipper Domains of the Transcription Factors GCN4 and c-Jun Have Ribonuclease Activity. Plos One. 5, 1-12.