Protein Information

General Information
MoonProt ID138
First appeared in release1.0
Name(s)birA biotin synthetase Bifunctional protein BirA Bifunctional ligase/repressor BirA Biotin operon repressor Biotin-[acetyl-CoA-carboxylase] ligase Biotin-protein ligase Biotin-[acetyl-CoA carboxylase] synthetase Gene Name: birA
UniProt IDP06709 (BIRA_ECOLI) Reviewed
GO termsGO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006464 cellular protein modification process GO:0009102 biotin biosynthetic process GO:0009305 protein biotinylation GO:0000166 nucleotide binding GO:0003677 DNA binding GO:0003824 catalytic activity GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity GO:0005524 ATP binding GO:0016874 ligase activity GO:0003677 DNA-binding GO:0006355 regulation of transcription, DNA-templated
Organisms for which functions have been demonstratedEscherichia coli
Sequence length321
FASTA sequence>sp|P06709|BIRA_ECOLI Bifunctional ligase/repressor BirA OS=Escherichia coli (strain K12) GN=birA PE=1 SV=1 MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionbirA biotin synthetase, enzyme biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
References for functionHoward, Peter K., Janet Shaw, and Anthony J. Otsuka. "Nucleotide sequence of the birA gene encoding the biotin operon represser and biotin holoenzyme synthetase functions of Escherichia coli." Gene 35.3 (1985): 321-331.
E.C. number6.3.4.15
Location of functional site(s)Binding site:112,183 DNA binding site:22-41
Cellular location of function
Comments
Function 2
Function descriptionbio operon repressor repressor activity depends on cellular concentration of biotin
References for functionBarker DF, Campbell AM. Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981 Mar 15. PMID: 6456358
E.C. numberN/A
Location of functional site(s)
Cellular location of functionbound to DNA
Comments