Protein Information

General Information
MoonProt ID147
First appeared in release1.0
Name(s)Transketolase 1 TktA Gene Name: tktA
UniProt IDP27302 (TKT1_ECOLI), Reviewed
GO termsGO:0008152 metabolic process GO:0009052 pentose-phosphate shunt, non-oxidative branch GO:0003824 catalytic activity GO:0004802 transketolase activity GO:0016740 transferase activity GO:0030145 manganese ion binding GO:0030976 thiamine pyrophosphate binding GO:0046872 metal ion binding GO:0003677 DNA-binding GO:0006351 transcription, DNA-templated
Organisms for which functions have been demonstratedEscherichia coli
Sequence length663
FASTA sequence>sp|P27302|TKT1_ECOLI Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptiontransketolase, enzyme Pentose Phosphate Pathway Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate <=> D-ribose 5-phosphate + D-xylulose 5-phosphate.
References for functionLois, Luisa Maria, et al. "Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis." Proceedings of the National Academy of Sciences95.5 (1998): 2105-2110.
E.C. number2.2.1.1
Location of functional site(s)Active site:411 Binding site:469,358,520,156,385,26,437,461,473,185,261,66 Metal binding site:185,155,187 Nucleotide binding site:114-116 Other functional site:261,26
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionTranscriptional regulator derepresses the marRAB multiple antibiotic resistance operon by binding to the MarR repressor a "trigger enzyme"
References for functionDomain F, Bina XR, Levy SB. Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol. 2007 Oct. PMID: 17850260
E.C. numberN/A
Location of functional site(s)
Cellular location of functionwith DNA
Comments