Protein Information

General Information
MoonProt ID163
First appeared in release1.0
Name(s)glutamine synthetase Glutamate--ammonia ligase Gene name: glnA
UniProt IDP12425 (GLNA_BACSU) Reviewed
GO termsGO:0006542 glutamine biosynthetic process GO:0006807 nitrogen compound metabolic process GO:0009399 nitrogen fixation GO:0000166 nucleotide binding GO:0003824 catalytic activity GO:0004356 glutamate-ammonia ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016874 ligase activity GO:0005737 cytoplasm GO:0005515 protein binding GO:0008134 transciption factor binding
Organisms for which functions have been demonstratedBacillus subtilis
Sequence length444
FASTA sequence>gi|349594718|gb|AEP90905.1| glutamine synthetase [Bacillus subtilis subsp. subtilis str. RO-NN-1] MAKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVNGSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFKSNEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionglutamine synthetase catalyzes the synthesis of glutamine from glutamate and ammonia ATP + L-glutamate + NH3 => ADP + phosphate + L-glutamine
References for functionDeuel, Thomas F., et al. "Bacillus subtilis glutamine synthetase purification and physical characterization." Journal of Biological Chemistry 245.20 (1970): 5195-5205.
E.C. number6.3.1.2
Location of functional site(s)Binding site:249,316,321,335,304,184,241,298 Metal binding site:333,134,132,189,245,196 Other functional site:62
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds to transcription factor TnrA, a global regulator of transcription, and prevents it from binding to DNA
References for functionWray LV, Zalieckas JM, Fisher SH. Bacillus subtilis glutamine synthetase controls gene expression through a protein-protein interaction with transcription factor TnrA. Cell. 2001 Nov 16. PMID: 11719184
E.C. numberN/A
Location of functional site(s)
Cellular location of functionbinding to DNA
Comments