Protein Information

General Information
MoonProt ID172
First appeared in release1.0
Name(s)glutamyl-prolyl tRNA synthetase Bifunctional glutamate/proline--tRNA ligase Bifunctional aminoacyl-tRNA synthetase Cell proliferation-inducing gene 32 protein Glutamatyl-prolyl-tRNA synthetase Glutamate--tRNA ligase Glutamyl-tRNA synthetase GluRS Proline--tRNA ligase Prolyl-tRNA synthetase Gene Name: EPRS
UniProt IDP07814 (SYEP_HUMAN), Reviewed
GO termsGO:0006412 translation GO:0006417 regulation of translation GO:0006418 tRNA aminoacylation for protein translation GO:0006424 glutamyl-tRNA aminoacylation GO:0006433 prolyl-tRNA aminoacylation GO:0006461 protein complex assembly GO:0008152 metabolic process GO:0010467 gene expression GO:0017148 negative regulation of translation GO:0043039 tRNA aminoacylation GO:0071346 cellular response to interferon-gamma GO:0000166 nucleotide binding GO:0003723 RNA binding GO:0003824 catalytic activity GO:0004812 aminoacyl-tRNA ligase activity GO:0004818 glutamate-tRNA ligase activity GO:0004827 proline-tRNA ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016874 ligase activity GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds GO:0035613 RNA stem-loop binding GO:0005737 cytoplasm GO:0005829 cytosol GO:0030529 ribonucleoprotein complex GO:0097452 GAIT complex GO:0006412 translation
Organisms for which functions have been demonstratedHomo sapiens
Sequence length1512
FASTA sequence>sp|P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens GN=EPRS PE=1 SV=5 MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionglutamyl-prolyl tRNA synthetase, enzyme an aminoacyl-tRNA synthetase catalyze the attachment of amino acids to cognate tRNAs ATP + L-glutamate + tRNA(Glu) => AMP + diphosphate + L-glutamyl-tRNA(Glu) ATP + L-proline + tRNA(Pro) => AMP + diphosphate + L-prolyl-tRNA(Pro)
References for functionSampath, Prabha, et al. "Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation." Cell119.2 (2004): 195-208.
E.C. number6.1.1.17 and 6.1.1.15
Location of functional site(s)Binding site:1152,398,1242,1276,211 Metal binding site:1495,1448,1497,1453 Nucleotide binding site:1152-1154,1163-1164,1237-1240,432-436
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptiontranslation inhibition part of GAIT complex: interferon (IFN)-gamma-activated inhibitor of translation silences ceruloplasmin mRNA translation
References for functionSampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL. Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation. Cell. 2004 Oct 15. PMID: 15479637
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments