Protein Information

General Information
MoonProt ID201
First appeared in release1.0
Name(s)Hexokinase-2 Hexokinase PII Hexokinase-B Gene Name:HXK2
UniProt IDP04807 (HXKB_YEAST), Reviewed
GO termsGO:0001302 replicative cell aging GO:0005975 carbohydrate metabolic process GO:0006000 fructose metabolic process GO:0006006 glucose metabolic process GO:0006013 mannose metabolic process GO:0006096 glycolytic process GO:0008152 metabolic process GO:0016310 phosphorylation GO:0019318 hexose metabolic process GO:0032445 fructose import GO:0046015 regulation of transcription by glucose GO:0046323 glucose import GO:0046835 carbohydrate phosphorylation GO:0000166 nucleotide binding GO:0003824 catalytic activity GO:0004396 hexokinase activity GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0016773 phosphotransferase activity, alcohol group as acceptor GO:0005634 nucleus GO:0005739 mitochondrion GO:0005829 cytosol GO:0005515 protein binding GO:0008134 transcription factor binding
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length486
FASTA sequence>sp|P04807|HXKB_YEAST Hexokinase-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HXK2 PE=1 SV=4 MVHLGPKKPQARKGSMADVPKELMQQIENFEKIFTVPTETLQAVTKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLRVVLVKLGGDRTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQFPQGISEPIPLGFTFSFPASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALINDTTGTLVASYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGKLSDDIPPSAPMAINCEYGSFDNEHVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDMYKQGFIFKNQDLSKFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGINTTVQERKLIRRLSELIGARAARLSVCGIAAICQKRGYKTGHIAADGSVYNRYPGFKEKAANALKDIYGWTQTSLDDYPIKIVPAEDGSGAGAAVIAALAQKRIAEGKSVGIIGA
Structure Information
PDB ID1IG8
Quaternary structure
Function 1
Function descriptionhexokinase, enzyme ATP + D-hexose => ADP + D-hexose 6-phosphate. Carbohydrate metabolism, hexose metabolism, glycolysis
References for function
E.C. number2.7.1.1
Location of functional site(s)binding site: 111, 158, 237, 269, 302; nucleotide binding: 86-91, 307-308, 344-348, 419-423
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionHxk2 interacts directly with Mig1, a transcriptional repressor that binds to promoters of genes that are repressed by glucose high glucose causes Hxk2 to move to the nucleus
References for functionAhuatzi D, Herrero P, de la, Moreno F. The glucose-regulated nuclear localization of hexokinase 2 in Saccharomyces cerevisiae is Mig1-dependent. J Biol Chem. 2004 Apr 2. PMID: 14715653. Herrero P, Mart?nezCampa C, Moreno F. The hexokinase 2 protein participates in regulatory DNA-protein complexes necessary for glucose repression of the SUC2 gene in Saccharomyces cerevisiae. FEBS Lett. 1998 Aug 28. PMID: 9738454. Rodr?guez A, De La, Herrero P, Moreno F. The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J. 2001 May 1. PMID: 11311123.
E.C. numberN/A
Location of functional site(s)
Cellular location of functionnucleus
Comments