Protein Information

General Information
MoonProt ID203
First appeared in release1.0
Name(s)Pyruvate carboxylase PCB Gene Name:PYC
UniProt IDQ8X1T3 (PYC_PICAN), Reviewed
GO termsGO:0006094 gluconeogenesis GO:0008152 metabolic process GO:0000166 nucleotide binding GO:0003677 DNA binding GO:0003824 catalytic activity GO:0004075 biotin carboxylase activity GO:0005524 ATP binding GO:0016874 ligase activity GO:0046872 metal ion binding GO:0005737 cytoplasm GO:0061077 chaperone-mediated protein folding
Organisms for which functions have been demonstratedHansenula poymorpha (Pichia angusta) (Yeast)
Sequence length1175
FASTA sequence>sp|Q8X1T3|PYC_PICAN Pyruvate carboxylase OS=Pichia angusta GN=PYC PE=3 SV=1 MAQVEDYSSLHRLRKNSEILSNANKILVANRGEIPIRIFRSAHELSMQTVAIYSHEDRLSMHRLKADEAYVIGARGQYSPVQAYLQIDEIINIALEHNVSMIHPGYGFLSENSEFARKVEDSGMIWIGPPHNVIDAVGDKVSARNLAGKCNVPVVPGTDGPIDSVEQAQEFVDKYGYPVIIKAAFGGGGRGMRVVREGESIADAFQRATSEAKTAFGNGTCFIERFLDKPKHIEVQLLADNYGNVIHLFERDCSVQRRHQKVVEIAPAKTLPVEVRDAILTDAVKLAKAANYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEVTGVDIVAAQIQIAAGASLQQLGLLQDKITTRGFAIQCRITTEDPAKNFQPDTGKIEVYRSSGGNGVRLDGGNGFAGAIISPHYDSMLVKCSTSGSNYEIARRKMIRALVEFRIRGVKTNIPFLLALLTHPTFVSGDCWTTFIDDTPSLFEMVQSKNRAQKLLSYLADLCVNGSSIKGQIGLPKLTRDADIPVIHDINGWDIDIKNTPPPESFRQYLLDYGPEQFANQIRAFDGCLIMDTTWRDAHQSLLATRVRTIDLLNIAPATAHAFRYAFALECWGGATFDVAMRFLHEDPWDRLRKLRKAVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDAGVDIFRVFDALNDLEQLKVGVDAVKKAGGVVEATVCYSGDMLKPGKKYNLKYYLETVDKIMEMGTHLLGIKDMAGTLKPAAAKLLISSIRKKYPSVPIHVHTHDSAGTGVITYVACALAGADVVDCAVNSMSGLTSQPSMSAFIAALDNEINTGITEQNAREIDAYWSEMRLLYSCFEADLKGPDPEVYNHEIPGGQLTNLLFQAQQVGLGEKWLETKKAYEEANMLLGDIVKVTPTSKVVGDLAQFMVSNKLSPKDVERLASELDFPDSVLDFFEGLMGTPYGGFPEPLRTNILAGKRRKLTRRPGLELEPFDLKKIKEELQSRFGNSITECDVASYNMYPKVFESFKKIQEKYGDLSVLPTRFFLAPPKLNEEISVEIEQGKTFVIKVMAIGDLSPQTGTREVYFEFNGEMRKVTVEDKLAAVETVTRPKADAHNPNEVGAPMAGVVIEVRVHPGVEVKKGDPLCVLSAMKMEMVISSPVSGRVGEVIVHENDSVDAGDLICKITK
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionPyruvate carboxylase, enzyme ATP + pyruvate + HCO3- => ADP + phosphate + oxaloacetate. Carbohydrate biosynthesis, gluconeogenesis
References for functionIshchuk, Olena P., et al. "Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose." FEMS yeast research 8.7 (2008): 1164-1174.
E.C. number6.4.1.1
Location of functional site(s)binding site: 140, 224, 259, 642, 902; active site: 316; metal binding: 570, 738, 768, 770
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionassembly of peroxisomal alcohol oxidase (AOX) may mediate FAD binding to AOX monomers in cytoplasm, which enables them to be transported to the peroxisome where they can assemble into the active octomeric form
References for functionOzimek P, van Dijk, Latchev K, Gancedo C, Wang DY, van der, Veenhuis M. Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase. Mol Biol Cell. 2003 Feb. PMID: 12589070. Ozimek PZ, Klompmaker SH, Visser N, Veenhuis M, van der. The transcarboxylase domain of pyruvate carboxylase is essential for assembly of the peroxisomal flavoenzyme alcohol oxidase. FEMS Yeast Res. 2007 Oct. PMID: 17316367.
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments