Protein Information

General Information
MoonProt ID207
First appeared in release1.0
Name(s)Bifunctional protein PutA Proline oxidase Delta-1-pyrroline-5-carboxylate dehydrogenase P5C dehydrogenase L-glutamate gamma-semialdehyde dehydrogenase Proline utilization A Transcriptional repressor Proline dehydrogenase Gene Name: putA ***This protein has two different enzyme functions at the C-terminal and N-terminal ends, which is probably due to gene fusion during evolution. We consider it to be a Moonlighting Protein because it has a transcription repressor function in addition to the enzyme functions.****
UniProt IDP09546 (PUTA_ECOLI), Reviewed
GO termsGO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006537 glutamate biosynthetic process GO:0006560 proline metabolic process GO:0006561 proline biosynthetic process GO:0006562 proline catabolic process GO:0008152 metabolic process GO:0010133 proline catabolic process to glutamate GO:0045892 negative regulation of transcription, DNA-templated GO:0055114 oxidation-reduction process GO:0000986 bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding GO:0001141 bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0003677 DNA binding GO:0003700 sequence-specific DNA binding transcription factor activity GO:0003824 catalytic activity GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity GO:0004657 proline dehydrogenase activity GO:0016491 oxidoreductase activity GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0043565 sequence-specific DNA binding GO:0050660 flavin adenine dinucleotide binding GO:0009898 cytoplasmic side of plasma membrane GO:0003677 DNA binding GO:0006351 transcription, DNA-templated
Organisms for which functions have been demonstratedEscherichia coli
Sequence length1320
FASTA sequence>sp|P09546|PUTA_ECOLI Bifunctional protein PutA OS=Escherichia coli (strain K12) GN=putA PE=1 SV=3 MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQLENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRPETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGKLVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEESDRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDLANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPREVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGSTEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAGQRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHIQTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYNRNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPFGGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAKYPVDAQLKAALTQPLNALREWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDALTQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDSDQLRALCEAVAARDGTIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
Structure Information
PDB ID4O8A 1TIW 1TJ0 1TJ1 2FZM 2FZN 4JNZ 1TJ2 3ITG 4JNY 3E2Q 3EWR 3E2S
Quaternary structure
Function 1
Function descriptionProline dehydrogenase / Proline oxidase, enzyme L-proline + a quinone => (S)-1-pyrroline-5-carboxylate + a quinol L-glutamate 5-semialdehyde + NAD+ + H2O => L-glutamate + NADH Amino-acid degradation, L-proline degradation into L-glutamate
References for functionRC Scarpulla and RL Soffer. (1978) Membrane-bound Proline Dehydrogenase from Escherichia coli, solubilization, purification and characterization. J. Biol. Chem. 253, 5997-6001. JM Wood. (1981) Genetics of L-Proline Utilization in Escherichia coli. J. Bacte. 146, 895-901.
E.C. number1.5.5.2 and 1.2.1.88
Location of functional site(s)active site: 883, 917
Cellular location of functionCytoplasmic side of membrane, peripheral membrane protein
Comments
Function 2
Function descriptiontranscriptional repressor of the put operon
References for functionJM Wood. (1981) Genetics of L-Proline Utilization in Escherichia coli. J. Bacte. 146, 895-901. PMID: 7016835
E.C. numberN/A
Location of functional site(s)
Cellular location of functionCytoplasm, bound to DNA
Comments