Protein Information

General Information
MoonProt ID209
First appeared in release1.0
Name(s)Cytosol aminopeptidase Aminopeptidase A/I Leucine aminopeptidase LAP Leucyl aminopeptidase Gene Name:pepA
UniProt IDP68767 (AMPA_ECOLI), Reviewed
GO termsGO:0006276 plasmid maintenance GO:0006310 DNA recombination GO:0006351 transcription, DNA-templated GO:0006508 proteolysis GO:0019538 protein metabolic process GO:0031564 transcription antitermination GO:0042150 plasmid recombination GO:0043171 peptide catabolic process GO:0001073 DNA binding transcription antitermination factor activity GO:0003677 DNA binding GO:0004177 aminopeptidase activity GO:0008233 peptidase activity GO:0008235 metalloexopeptidase activity GO:0016787 hydrolase activity GO:0030145 manganese ion binding GO:0046872 metal ion binding GO:0005622 intracellular GO:0005737 cytoplasm GO:0003677 DNA binding GO:0006351 transcription, DNA-templated
Organisms for which functions have been demonstratedEscherichia coli
Sequence length503
FASTA sequence>sp|P68767|AMPA_ECOLI Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLNDTGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFNVPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNVITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDSGGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRPGDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHITGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITAGCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE
Structure Information
PDB ID1GYT
Quaternary structure
Function 1
Function descriptionaminopeptidase, enzyme removes amino-terminal amino acid, preferentially if it is Leu
References for functionCharlier D, Kholti A, Huysveld N, Gigot D, Maes D, ThiaToong TL, Glansdorff N. Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. J Mol Biol. 2000 Sep 15. PMID: 10970742
E.C. number3.4.11.1 and 3.4.11.10
Location of functional site(s)metal binding: 270, 275, 293, 352, 354; active site: 282, 356
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptiontranscriptional repressor binds DNA, binds car operator DNA represses the carAB operon
References for functionCharlier D, Kholti A, Huysveld N, Gigot D, Maes D, ThiaToong TL, Glansdorff N. Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. J Mol Biol. 2000 Sep 15. PMID: 10970742
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm, binds to DNA
Comments