Protein Information

General Information
MoonProt ID212
First appeared in release1.0
Name(s)dihydroxyacetone kinase PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK Gene Name:dhaK
UniProt IDP76015 (DHAK_ECOLI), Reviewed
GO termsGO:0006071 glycerol metabolic process GO:0006974 cellular response to DNA damage stimulus GO:0016310 phosphorylation GO:0019563 glycerol catabolic process GO:0042182 ketone catabolic process GO:0046365 monosaccharide catabolic process GO:0004371 glycerone kinase activity GO:0005515 protein binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0047324 phosphoenolpyruvate-glycerone phosphotransferase activity GO:0005829 cytosol GO:0005515 protein binding GO:0008134 transcription factor binding
Organisms for which functions have been demonstratedEscherichia coli
Sequence length356
FASTA sequence>sp|P76015|DHAK_ECOLI PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK OS=Escherichia coli (strain K12) GN=dhaK PE=1 SV=2 MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAGKVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLIIKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIHGEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGDRVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLKVDDETLALWDAPVHTPALNWGK
Structure Information
PDB ID1OI2 1OI3 1UID 3PNK 3PNL 4LRY 3PNO 3PNQ 3PNM 1UOE
Quaternary structure
Function 1
Function descriptionsubunit of dihydroxyacetone kinase, enzyme phosphorylates dihydroxyacetone contains the Dha binding site
References for functionGutknecht, Regula, et al. "The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor." The EMBO journal 20.10 (2001): 2480-2486.
E.C. number2.7.-.-
Location of functional site(s)active site: 56, 218; binding site: 104, 109
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptioncorepressor of DhaR transcription activator binds to DhaR affects transcription of the dhaKLM operon
References for functionB?chler C, Schneider P, B?hler P, Lustig A, Erni B. Escherichia coli dihydroxyacetone kinase controls gene expression by binding to transcription factor DhaR. EMBO J. 2005 Jan 26. PMID: 15616579
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments