Protein Information

General Information
MoonProt ID218
First appeared in release1.0
Name(s)Glycogen debranching enzyme TreX Gene Name:treX
UniProt IDQ7LX99 (Q7LX99_SULSO), Unreviewed
GO termsGO:0005975 carbohydrate metabolic process GO:0005980 glycogen catabolic process GO:0008152 metabolic process GO:0003824 catalytic activity GO:0004133 glycogen debranching enzyme activity GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds GO:0016787 hydrolase activity GO:0016798 hydrolase activity, acting on glycosyl bonds GO:0043169 cation binding GO:0090599 alpha-glucosidase activity
Organisms for which functions have been demonstratedSulfologus solfataricus
Sequence length718
FASTA sequence>tr|Q7LX99|Q7LX99_SULSO Glycogen debranching enzyme (TreX) OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=treX PE=4 SV=1 MALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEIIEVKNKTGDIWHVFVPGLRPGQLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWNDAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDDEDFIKGKKVPLKDTVIYEVHVKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTNYWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEPWDVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVSYNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLEGSVMDEINMYGERIADDSFLIILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL
Structure Information
PDB ID2VNC 2VR5 2VUY
Quaternary structure
Function 1
Function descriptionalpha-1,4-transferase, enzyme
References for functionWoo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17. PMID: 18703518
E.C. number3.2.1.-
Location of functional site(s)
Cellular location of functioncytoplasm
Comments"TreX exhibits two different active-site configurations depending on its oligomeric state. The N terminus of one subunit is located at the active site of the other molecule, resulting in a reshaping of the active site in the tetramer. This is accompanied by a large shift in the "flexible loop" (amino acids 399-416), creating connected holes inside the tetramer." from Woo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17;283(42):28641-8. doi: 10.1074/jbc.M802560200. PMID: 18703518
Function 2
Function descriptionalpha-1,6-glucosidase, enzyme
References for functionWoo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17. PMID: 18703518
E.C. number 3.2.1.33
Location of functional site(s)
Cellular location of functioncytoplasm
Comments