Protein Information

General Information
MoonProt ID222
First appeared in release1.0
Name(s)Dihydrofolate reductase DHFR Gene Name:DHFR
UniProt IDP00374 (DYR_HUMAN), Reviewed
GO termsGO:0000082 G1/S transition of mitotic cell cycle GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0000278 mitotic cell cycle GO:0006545 glycine biosynthetic process GO:0006730 one-carbon metabolic process GO:0006766 vitamin metabolic process GO:0006767 water-soluble vitamin metabolic process GO:0009165 nucleotide biosynthetic process GO:0031427 response to methotrexate GO:0044281 small molecule metabolic process GO:0046209 nitric oxide metabolic process GO:0046653 tetrahydrofolate metabolic process GO:0046654 tetrahydrofolate biosynthetic process GO:0046655 folic acid metabolic process GO:0050999 regulation of nitric-oxide synthase activity GO:0055114 oxidation-reduction process GO:0003723 RNA binding GO:0003729 mRNA binding GO:0004146 dihydrofolate reductase activity GO:0008144 drug binding GO:0016491 oxidoreductase activity GO:0050661 NADP binding GO:0005575 cellular_component GO:0005654 nucleoplasm GO:0005829 cytosol GO:0006412 translation GO:0003729 mRNA binding
Organisms for which functions have been demonstratedHomo sapiens
Sequence length187
FASTA sequence>sp|P00374|DYR_HUMAN Dihydrofolate reductase OS=Homo sapiens GN=DHFR PE=1 SV=2 MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Structure Information
PDB ID1MVS, 1MVT, 3FS6, 3GYF, 2W3A, 2W3B, 2W3M, 2M6J, 4M6K, 4M6L, 3F8Y, 3GI2, 1DHF, 2DHF, 1KMS, 1KMV, 1PD8, 1PD9, 1PDB, 1S3U, 1S3V, 1S3W, 1U72, 1YHO, 2C2S, 2C2T, 1C2T, 1DRF, 1HFR, 1OHJ, 1OHK, 3GHW, 3NXR, 3NXT, 3NXO, 3L3R, 1DLR, 1DLS, 1U71, 1HFQ, 3F91, 1BOZ, 3
Quaternary structure
Function 1
Function descriptiondihydrofolate reductase, enzyme 5,6,7,8-tetrahydrofolate + NADP+ => 7,8-dihydrofolate + NADPH tetrahydrofolate biosynthesis
References for function
E.C. number1.5.1.3
Location of functional site(s)binding site: 10, 65, 71; nucleotide binding: 16-22, 55-57, 77-79, 117-124
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds DHFR mRNA regulation of DHFR synthesis methotrexate inhibits interaction
References for functionChu E, Takimoto CH, Voeller D, Grem JL, Allegra CJ. Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro. Biochemistry. 1993 May 11. PMID: 8490020
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments