Protein Information

General Information
MoonProt ID227
First appeared in release1.0
Name(s)Threonyl-tRNA synthetase Threonine-tRNA ligase ThrRS Gene Name:thrS
UniProt IDP0A8M3 (SYT_ECOLI), Reviewed
GO termsGO:0006412 translation GO:0006417 regulation of translation GO:0006418 tRNA aminoacylation for protein translation GO:0006435 threonyl-tRNA aminoacylation GO:0043039 tRNA aminoacylation GO:0045947 negative regulation of translational initiation GO:0000166 nucleotide binding GO:0000900 translation repressor activity, nucleic acid binding GO:0003723 RNA binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004812 GO:0004829 threonine-tRNA ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0008144 drug binding GO:0008270 zinc ion binding GO:0016874 ligase activity GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds GO:0046872 metal ion binding GO:0005737 cytoplasm GO:0003723 RNA binding GO:0006412 translation GO:0003729 mRNA binding
Organisms for which functions have been demonstratedEscherichia coli
Sequence length642
FASTA sequence>sp|P0A8M3|SYT_ECOLI Threonine--tRNA ligase OS=Escherichia coli (strain K12) GN=thrS PE=1 SV=1 MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGELVDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSILDENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQEIRSRSLKQLEE
Structure Information
PDB ID1QF6 4HWO 4HWP 4HWR 4HWS 1EVK 1EVL 1FYF 1KOG
Quaternary structure
Function 1
Function descriptionThreonine-tRNA ligase, enzyme ATP + L-threonine + tRNA(Thr) => AMP + diphosphate + L-threonyl-tRNA(Thr)
References for functionMayaux, Jean-Francois, et al. "Structural and transcriptional evidence for related thrS and infC expression." Proceedings of the National Academy of Sciences 80.20 (1983): 6152-6156.
E.C. number6.1.1.3
Location of functional site(s)binding site: 309, 325, 363, 375, 376, 379, 381, 462, 484, 517, 520, 589, 609, 615; metal binding: 334, 385, 511; nucleotide binding: 363-365; 479-480
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds mRNA binds mRNA encoding threonyl-tRNA synthetase, controls expression of its own gene at the translational level
References for functionSpringer M, Plumbridge JA, Butler JS, Graffe M, Dondon J, Mayaux JF, Fayat G, Lestienne P, Blanquet S, GrunbergManago M. Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in vivo. J Mol Biol. 1985 Sep 5. PMID: 3930755
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments