Protein Information

General Information
MoonProt ID235
First appeared in release1.0
Name(s)Alanyl-tRNA synthetase Alanine--tRNA ligase AlaRS Gene Name: alaS
UniProt IDP00957 (SYA_ECOLI), Reviewed
GO termsGO:0006412 translation GO:0006419 alanyl-tRNA aminoacylation GO:0006450 regulation of translational fidelity GO:0043039 tRNA aminoacylation GO:0045892 negative regulation of transcription, DNA-templated GO:0000049 tRNA binding GO:0000166 nucleotide binding GO:0001141 bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription GO:0002161 aminoacyl-tRNA editing activity GO:0003676 nucleic acid binding GO:0003723 RNA binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004813 alanine-tRNA ligase activity GO:0005524 ATP binding GO:0008270 zinc ion binding GO:0016874 ligase activity GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds GO:0042802 identical protein binding GO:0046872 metal ion binding GO:0005829 cytosol GO:0016020 membrane GO:0003677 DNA binding GO:0006351 transcription, DNA-templated
Organisms for which functions have been demonstratedEscherichia coli
Sequence length876
FASTA sequence>sp|P00957|SYA_ECOLI Alanine--tRNA ligase OS=Escherichia coli (strain K12) GN=alaS PE=1 SV=2 MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ
Structure Information
PDB ID3HXU 3HXV 3HXW 3HXX 3HXY 3HXZ 3HY0 3HY1
Quaternary structure
Function 1
Function descriptionAlanyl-tRNA synthetase, enzyme attaches alanine to tRNA(Ala) ATP + L-alanine + tRNA(Ala) => AMP + diphosphate + L-alanyl-tRNA(Ala)
References for functionChen, Zhang-Qun, et al. "Reactions of the sulfhydryl groups of alanyl-tRNA synthetase." Archives of biochemistry and biophysics233.2 (1984): 611-616.
E.C. number6.1.1.7
Location of functional site(s)metal binding: 564, 568, 666, 670
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptiontranscription regulator represses transcription of its own gene increased repression with increased alanine concentrations binds DNA
References for functionPutney SD, Schimmel P. An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature. 1981 Jun 25. PMID: 6264314
E.C. numberN/A
Location of functional site(s)
Cellular location of functionbinding to DNA
Comments