Protein Information

General Information
MoonProt ID236
First appeared in release1.0
Name(s)Leucyl-tRNA synthetase Leucine--tRNA ligase, mitochondrial ymLeuRS LeuRS Gene Name: NAM2
UniProt IDP11325
GO termsGO:0000372 Group I intron splicing GO:0006412 translation GO:0006418 tRNA aminoacylation for protein translation GO:0006429 leucyl-tRNA aminoacylation GO:0006450 regulation of translational fidelity GO:0032543 mitochondrial translation GO:0000166 nucleotide binding GO:0002161 aminoacyl-tRNA editing activity GO:0003729 mRNA binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004823 leucine-tRNA ligase activity GO:0005524 ATP binding GO:0016874 ligase activity GO:0005739 mitochondrion GO:0005759 mitochondrial matrix GO:0043484 regulation of RNA splicing GO:0000372 Group I intron splicing
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length894
FASTA sequence>sp|P11325|SYLM_YEAST Leucine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM2 PE=1 SV=1 MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionLeucyl-tRNA synthetase, enzyme ATP + L-leucine + tRNA(Leu) => AMP + diphosphate + L-leucyl-tRNA(Leu) protein synthesis
References for functionTzagoloff, Alexander, et al. "Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl-and methionyl-tRNA synthetases of Escherichia coli." Journal of Biological Chemistry263.2 (1988): 850-856.
E.C. number6.1.1.4
Location of functional site(s)binding site: 649
Cellular location of functionMitochondrial matrix.
Comments
Function 2
Function descriptionintron splicing, RNA splicing group I intron splicing
References for functionSarkar J, Poruri K, Boniecki MT, McTavish KK, Martinis SA. Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing. J Biol Chem. 2012 Apr 27. PMID: 22383526
E.C. numberN/A
Location of functional site(s)
Cellular location of functionnucleus
Comments