Protein Information

General Information
MoonProt ID237
First appeared in release1.0
Name(s)Leucyl-tRNA synthetase Leucine--tRNA ligase, cytoplasmic LeuRS Gene Name: LARS
UniProt IDQ9P2J5 (SYLC_HUMAN), Reviewed
GO termsGO:0006412 translation GO:0006418 tRNA aminoacylation for protein translation GO:0006429 leucyl-tRNA aminoacylation GO:0006450 regulation of translational fidelity GO:0010467 gene expression GO:0000166 nucleotide binding GO:0002161 aminoacyl-tRNA editing activity GO:0004812 aminoacyl-tRNA ligase activity GO:0004823 leucine-tRNA ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016874 ligase activity GO:0005737 cytoplasm GO:0005829 cytosol GO:0043087 regulation of GTPase activity GO:0005515 protein binding
Organisms for which functions have been demonstratedHomo sapiens
Sequence length1176
FASTA sequence>sp|Q9P2J5|SYLC_HUMAN Leucine--tRNA ligase, cytoplasmic OS=Homo sapiens GN=LARS PE=1 SV=2 MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH
Structure Information
PDB ID2WFD
Quaternary structure
Function 1
Function descriptionLeucyl-tRNA synthetase, enzyme attaches leucine to its cognate tRNA ATP + L-leucine + tRNA(Leu) => AMP + diphosphate + L-leucyl-tRNA(Leu) protein synthesis
References for functionVander Horn, P. B., and STANLEY A. Zahler. "Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis." Journal of bacteriology 174.12 (1992): 3928-3935.
E.C. number6.1.1.4
Location of functional site(s)binding site: 719
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionGTPase-activating protein (GAP) senses intracellular leucing concentration and binds to Rag GTPase Rag signals to mTORC1 promotes translocation of mTORC1 to the lysosomal membrane
References for functionHan JM, Jeong SJ, Park MC, Kim G, Kwon NH, Kim HK, Ha SH, Ryu SH, Kim S. Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway. Cell. 2012 Apr 13. PMID: 22424946
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments