Protein Information

General Information
MoonProt ID239
First appeared in release1.0
Name(s)Lysyl-tRNA synthetase Lysine-tRNA ligase Gene Name: Kars
UniProt IDQ5XIM7 (Q5XIM7_RAT), Unreviewed
GO termsGO:0006418 tRNA aminoacylation for protein translation GO:0006430 lysyl-tRNA aminoacylation GO:0015966 diadenosine tetraphosphate biosynthetic process GO:0000049 tRNA binding GO:0000166 nucleotide binding GO:0003676 nucleic acid binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004824 lysine-tRNA ligase activity GO:0005524 ATP binding GO:0016597 amino acid binding GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0015630 microtubule cytoskeleton GO:0017101 aminoacyl-tRNA synthetase multienzyme complex GO:0015966 diadenosine tetraphosphate biosynthetic process. GO:0015965 diadenosine tetraphosphate metabolic process
Organisms for which functions have been demonstratedRattus norvegicus
Sequence length626
FASTA sequence>tr|Q5XIM7|Q5XIM7_RAT Lysine--tRNA ligase OS=Rattus norvegicus GN=Kars PE=2 SV=1 MLMQAAVRLVRGSLRQTSWAEWGQRELRLGQLAPFTTLRKDKPLSDRRSELKRRLKAEKKLAEKEAKQKELSEKQLNQTTAAAATNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVSGEDPYPHKFHVDISLTQFIQEYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIGVEGNPGKTKKGELSIVPREMTLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVRSITGSYKITYHPDGPEGQAYEIDFTPPFRRISMVEELEKVLGVKLPETSLFETEETRKILDDICVARAVECPPPRTTARLLDKLVGEFLEVTCISPTFICDHPQIMSPLAKWHRCKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVTMFLTDSNNIKEVLLFPAMKPEDKKETATATETPESTEASPSV
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionLysyl-tRNA synthetase, enzyme ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys) protein synthesis
References for functionHilderman, R. H., et al. "Characterization of a homogeneous arginyl-and lysyl-tRNA synthetase complex isolated from rat liver. Kinetic mechanism for lysyl-tRNA synthetase." Journal of Biological Chemistry 258.22 (1983): 13592-13596.
E.C. number6.1.1.6
Location of functional site(s)binding site: 306, 330, 368, 370, 526, 530; nucleotide binding: 352-354, 360-361, 523-524, 579-582
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionsynthesis of diadenosine tetraphosphate (diadenosine-5',5'''-P1,P4-tetraphosphate, Ap(4)A, AppppA) Ap4A then regulates transcription of MITF target genes
References for functionWahab SZ, Yang DC. Synthesis of diadenosine 5',5''' -P1,P4-tetraphosphate by lysyl-tRNA synthetase and a multienzyme complex of aminoacyl-tRNA synthetases from rat liver. J Biol Chem. 1985 May 10. PMID: 3988754. YannayCohen N, CarmiLevy I, Kay G, Yang CM, Han JM, Kemeny DM, Kim S, Nechushtan H, Razin E. LysRS serves as a key signaling molecule in the immune response by regulating gene expression. Mol Cell. 2009 Jun 12. PMID: 19524539. YannayCohen N, Razin E. Translation and transcription: the dual functionality of LysRS in mast cells. Mol Cells. 2006 Oct 31. PMID: 17085962.
E.C. number
Location of functional site(s)
Cellular location of functionnucleus
Comments