Protein Information

General Information
MoonProt ID244
First appeared in release1.0
Name(s)Lysyl-tRNA synthetase Lysine-tRNA ligase LysRS Gene Name: KARS
UniProt IDQ15046 (SYK_HUMAN), Reviewed
GO termsGO:0006412 translation GO:0006418 tRNA aminoacylation for protein translation GO:0006430 lysyl-tRNA aminoacylation GO:0008033 tRNA processing GO:0008219 cell death GO:0010467 gene expression GO:0015966 diadenosine tetraphosphate biosynthetic process GO:0016032 viral process GO:0000049 tRNA binding GO:0000166 nucleotide binding GO:0003676 nucleic acid binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004824 lysine-tRNA ligase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016597 amino acid binding GO:0016874 ligase activity GO:0046872 metal ion binding GO:0005576 extracellular region GO:0005634 nucleus GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0005759 mitochondrial matrix GO:0005829 cytosol GO:0005886 plasma membrane GO:0016020 membrane GO:0017101 aminoacyl-tRNA synthetase multienzyme complex GO: 0005125 cytokine activity
Organisms for which functions have been demonstratedHomo sapiens
Sequence length597
FASTA sequence>sp|Q15046|SYK_HUMAN Lysine--tRNA ligase OS=Homo sapiens GN=KARS PE=1 SV=3 MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV
Structure Information
PDB ID4DPG 3BJU
Quaternary structure
Function 1
Function descriptionLysyl-tRNA synthetase, enzyme ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys) protein synthesis
References for functionTolkunova, Elena, et al. "The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript." Journal of Biological Chemistry 275.45 (2000): 35063-35069.
E.C. number6.1.1.6
Location of functional site(s)binding site: 277, 301, 339, 341, 497, 501; nucleotide binding: 323-325, 331-332, 494-495, 550-553
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptioncytokine binds to macrophages and peripheral blood mononuclear cells increases TNF-alpha production by target cells increases target cell migration
References for functionPark SG, Kim HJ, Min YH, Choi EC, Shin YK, Park BJ, Lee SW, Kim S. Human lysyl-tRNA synthetase is secreted to trigger proinflammatory response. Proc Natl Acad Sci U S A. 2005 May 3. PMID: 15851690
E.C. numberN/A
Location of functional site(s)
Cellular location of functionextracellular, secreted
Comments