Protein Information

General Information
MoonProt ID3
First appeared in release1.0
Name(s)Structural maintenance of chromosome protein 3 Chondroitin sulfate proteoglycan 6 Chromosome segregation protein SmcD Basement membrane-associated chondroitin proteoglycan Bamacan Mad member-interacting protein 1 SMC protein 3 SMC-3 SMC3 Gene Name: Smc3
UniProt IDQ9CW03 (SMC3_MOUSE), Reviewed
GO termsGO:0006275 regulation of DNA replication GO:0006281 DNA repair GO:0006974 cellular response to DNA damage stimulus GO:0007049 cell cycle GO:0007052 mitotic spindle organization GO:0007067 mitosis GO:0007126 meiotic nuclear division GO:0007165 signal transduction GO:0019827 stem cell maintenance GO:0032876 negative regulation of DNA endoreduplication GO:0051276 chromosome organization GO:0051301 cell division GO:0000166 nucleotide binding GO:0003682 chromatin binding GO:0005515 protein binding GO:0005524 ATP binding GO:0036033 mediator complex binding GO:0045502 dynein binding GO:0046982 protein heterodimerization activity GO:0000775 chromosome, centromeric region GO:0000785 chromatin GO:0000800 lateral element GO:0000922 spindle pole GO:0005634 nucleus GO:0005654 nucleoplasm GO:0005694 chromosome GO:0005737 cytoplasm GO:0016363 nuclear matrix GO:0030893 meiotic cohesin complex GO:0034991 nuclear meiotic cohesin complex GO: 0005604 basement membrane
Organisms for which functions have been demonstratedMus musculus (Mouse)
Sequence length1217
FASTA sequence>sp|Q9CW03|SMC3_MOUSE Structural maintenance of chromosomes protein 3 OS=Mus musculus GN=Smc3 PE=1 SV=2 MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPKFNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDLEKKQQLLRAATGKAILNGIDSINKVLEHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFYHIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKEHMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG"
Structure Information
PDB ID2WD5
Quaternary structureHINGE HETERODIMER
Function 1
Function descriptionSMC3 interacts with SMC1 and other non-Smc subunits like Scc3 and Scc1 (also called Rad21) to form a cohesion complex, called "cohesin," that maintains proper sister chromatid cohesion throughout the cell cycle and during mitosis to ensure accurate chromosome segregation. Each Smc heterodimer associates with non-Smc subunits to form functional Smc complexes.
References for functionWu N, Yu H. The Smc complexes in DNA damage response. Cell Biosci. 2012 Feb 27;2:5. doi: 10.1186/2045-3701-2-5. PMID: 22369641. Darwiche N, Freeman LA, Strunnikov A. Characterization of the components of the putative mammalian sister chromatid cohesion complex. Gene. 1999 Jun 11. PMID: 10375619.
E.C. numberN/A
Location of functional site(s)Nucleotide binding site:32-39
Cellular location of functionnucleus, associated with chromosomes
Comments
Function 2
Function descriptionBamacan is a proteoglycan, and is a component of a component of the basement membrane in the Engelbreth-Holm-Swarm tumor matrix, the renal mesangial matrix, and the basement membrane of other tissues. Bamacan is involved in the control of cell growth and transformation
References for functionGhiselli G, Siracusa LD, Iozzo RV. Complete cDNA cloning, genomic organization, chromosomal assignment, functional characterization of the promoter, and expression of the murine Bamacan gene. J Biol Chem. 1999 Jun 11;274(24):17384-93.PMID: 10358101. Couchman JR, Kapoor R, Sthanam M, Wu RR. Perlecan and basement membrane-chondroitin sulfate proteoglycan (bamacan) are two basement membrane chondroitin/dermatan sulfate proteoglycans in the Engelbreth-Holm-Swarm tumor matrix. J Biol Chem. 1996 Apr 19;271(16):9595-602. PMID: 8621634.
E.C. numberN/A
Location of functional site(s)
Cellular location of functionbasement membrane, extracellular matrix
CommentsGlycanation sites (Ser-Gly) are present at residues 36, 249, 1073, 1081, and 1116, where attachment of glycosaminoglycan side GAG chains is possible There is a P-loop motif starting at residue 32 which is an ATP binding site, and a DA box motif starting at residue 1114 which is a DNA binding site. These are highly conserved in SMC proteins as well.