Protein Information

General Information
MoonProt ID305
First appeared in release2.0
Name(s)aldolase A, fructose-1,6-bisphosphate (fru 1,6?P2) aldolase, Fructose-bisphosphate aldolase A Gene: ALDOA
UniProt IDP00883 (ALDOA_RABIT)
GO termsGO:0006096 glycolytic process GO:0051289 protein homotetramerization GO:0003824 catalytic activity GO:0004332 fructose-bisphosphate aldolase activity GO:0005515 protein binding GO:0016829 lyase activity GO:0005737 cytoplasm GO:0031430 M band GO:0031674 I band GO:0005515 protein binding
Organisms for which functions have been demonstratedOryctolagus cuniculus (Rabbit)
Sequence length364 amino acids
FASTA sequence>sp|P00883|ALDOA_RABIT Fructose-bisphosphate aldolase A OS=Oryctolagus cuniculus GN=ALDOA PE=1 SV=2 MPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFISNHAY
Structure Information
PDB ID1ZAH, 3B8D, 1EWE, 2QUU, 3DFN, 6ALD, 3DFQ, 1EX5, 1ADO, 1EWD
Quaternary structure
Function 1
Function descriptionaldolase, cleavage of carbon-carbon bonds, in glycolysis, gluconeogenesis, and fructose metabolism, D-fructose 1,6-bisphosphate => dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate.
References for functionRitterson Lew C, Tolan DR. 2013. Aldolase sequesters WASP and affects. WASP/Arp2/3-stimulated actin dynamics. J Cell Biochem 114:1928-1939. PMID: 23495010
E.C. number4.1.2.13
Location of functional site(s)binding site: 43, 304; site: 73, 108, 147, 362, 364; active site: 188, 230
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionsequesters WASP, Wiskott–Aldrich Syndrome Protein, which is involved in controlling actin dynamics, inihibits actin polymerization, only binds to active from of WASP, inhibits WA?stimulated Arp2/3?dependent actin?polymerization reaction
References for functionRitterson Lew C, Tolan DR. 2013. Aldolase sequesters WASP and affects. WASP/Arp2/3-stimulated actin dynamics. J Cell Biochem 114:1928-1939. PMID: 23495010
E.C. number
Location of functional site(s)direct binding of aldolase to the acidic portion of the WA domain
Cellular location of functioncytoplasm
Commentsused mutations that knock out either catalytic activity or actin binding to show the two functions were separable, Ritterson Lew C, Tolan DR. 2013. Aldolase sequesters WASP and affects. WASP/Arp2/3-stimulated actin dynamics. J Cell Biochem 114:1928-1939. PMID: 23495010