Protein Information

General Information
MoonProt ID327
First appeared in release2.0
Name(s)eIF3 subunit g, Eukaryotic translation initiation factor 3 subunit G Gene: EIF3G
UniProt IDO75821 (EIF3G_HUMAN)
GO termsGO:0001731 formation of translation preinitiation complex GO:0006412 translation GO:0006413 translational initiation GO:0006446 regulation of translational initiation GO:0010467 gene expression GO:0044267 cellular protein metabolic process GO:0000166 nucleotide binding GO:0003676 nucleic acid binding GO:0003723 RNA binding GO:0003743 translation initiation factor activity GO:0005515 protein binding GO:0044822 poly(A) RNA binding GO:0003743 translation initiation factor activity GO:0005737 cytoplasm GO:0005829 cytosol GO:0005852 eukaryotic translation initiation factor 3 complex GO:0016282 eukaryotic 43S preinitiation complex GO:0033290 eukaryotic 48S preinitiation complex GO:0048471 perinuclear region of cytoplasm GO:0005634 nucleus GO:0003723 RNA binding GO:0006412 translation GO:0048027 mRNA 5-UTR binding
Organisms for which functions have been demonstratedhuman, homo sapiens
Sequence length320 amino acids
FASTA sequence>gi|49472822|ref|NP_003746.2| eukaryotic translation initiation factor 3 subunit G [Homo sapiens] MPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPEPELLPGAPLPPPKEVINGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATTTVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQKELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDGASRRGESMQPNRRADDNATIRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGVSGFGYDHLILNVEWAKPSTN
Structure Information
PDB IDpartial 2CQ0, fragment 2MJC
Quaternary structurepart of 13 subunits (a-m) complex, 800 kilodaltons
Function 1
Function descriptionpart of eIF3 13-subunit complex that organizes initiation factor and ribosome interactions required for productive translation, protein scaffold for formation of initiation complexes, binds to proteins of small ribosomal subunit
References for functionJackson RJ, Hellen CU, Pestova TV. The mechanism of eukaryotic translation initiation and principles of its regulation. Nat Rev Mol Cell Biol. 2010 Feb;11(2):113-27. doi: 10.1038/nrm2838. PMID: 20094052
E.C. number
Location of functional site(s)
Cellular location of function
Commentsoverall complex is phosphorylated: Andaya A, Villa N, Jia W, Fraser CS, Leary JA. Phosphorylation stoichiometries of human eukaryotic initiation factors. Int J Mol Sci. 2014 Jun 27;15(7):11523-38. doi: 10.3390/ijms150711523. PMID: 24979134
Function 2
Function descriptionbinds to 5' untranslated regions of specific mRNA for translational activation, subunits a, b, d, g, binding site on mRNA for c-Jun is required for efficient translation of c-JUN
References for function25849773
E.C. number
Location of functional site(s)
Cellular location of function
Commentsbinds mRNA stem-loops, with different modes of stem-loop binding to affect translation activation or repression, binds directly to c-JUN mRNA
Third Function
Function descriptionbinds to 5' untranslated regions of specific mRNA for translational repression, removing binding site on mRNA for BTG1 caused an increase in its translation
References for functionLee AS, Kranzusch PJ, Cate JH. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature. 2015 Jun 4;522(7554):111-4. doi: 10.1038/nature14267. PMID: 25849773
E.C. number
Location of functional site(s)
Cellular location of function
Commentsbinds mRNA stem-loops, with different modes of stem-loop binding to affect translation activation or repression, needs addtional factors for binding to BTG1 mRNA