Protein Information

General Information
MoonProt ID347
First appeared in release2.0
Name(s)GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Gene: Gapdh
UniProt IDP04797 (G3P_RAT)
GO termsGO:0000226 microtubule cytoskeleton organization GO:0005975 carbohydrate metabolic process GO:0006006 glucose metabolic process GO:0006094 gluconeogenesis GO:0006096 glycolytic process GO:0006417 regulation of translation GO:0006915 apoptotic process GO:0006915 apoptotic process GO:0035606 peptidyl-cysteine S-trans-nitrosylation GO:0050821 protein stabilization GO:0051402 neuron apoptotic process GO:0055114 oxidation-reduction process GO:0060359 response to ammonium ion GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0005515 protein binding GO:0008017 microtubule binding GO:0016491 oxidoreductase activity GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0016740 transferase activity GO:0035605 peptidyl-cysteine S-nitrosylase activity GO:0050661 NADP binding GO:0051287 NAD binding GO:0005634 nucleus GO:0005737 cytoplasm GO:0005829 cytosol GO:0005856 cytoskeleton GO:0015630 microtubule cytoskeleton GO:0097452 GAIT complex
Organisms for which functions have been demonstratedRattus norvegicus (rat)
Sequence length333 amino acids
FASTA sequence>sp|P04797|G3P_RAT Glyceraldehyde-3-phosphate dehydrogenase OS=Rattus norvegicus GN=Gapdh PE=1 SV=3 MVKVGVNGFGRIGRLVTRAAFSCDKVDIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQAAEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE
Structure Information
PDB IDNone, but structures of homologues are available
Quaternary structure
Function 1
Function descriptionGAPDH, converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate, enzyme in glycolysis, glyceraldehyde 3-phosphate + phosphate + NAD+ => 3-phospho-D-glyceroyl phosphate + NADH
References for functionFort, Ph, et al. "Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family." Nucleic Acids Research 13.5 (1985): 1431-1442.
E.C. number1.2.1.12
Location of functional site(s)binding site: 33, 78, 120, 180, 232, 314; active site: 150; site: 177; nucleotide binding: 11-12
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds to Siah1, involved in apoptosis
References for function15951807
E.C. number
Location of functional site(s)
Cellular location of functionnucleus
Commentswhen GAPDH is S-nitrosylated, increased binding to Siah1 and move to nucleus, some evidence that this function is also in mice and humans