Protein Information

General Information
MoonProt ID348
First appeared in release2.0
Name(s)GAPDH, Tdh3, Glyceraldehyde-3-phosphate dehydrogenase 3, GPD3 Gene: TDH3
UniProt IDP00359 (G3P3_YEAST)
GO termsGO:0006006 glucose metabolic process GO:0006094 gluconeogenesis GO:0006096 glycolytic process GO:0006915 apoptotic process GO:0055114 oxidation-reduction process GO:0072593 reactive oxygen species metabolic process GO:0003723 RNA binding GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0016491 oxidoreductase activity GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor GO:0050661 NADP binding GO:0051287 NAD binding GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0005811 lipid particle GO:0009277 fungal-type cell wall GO:0005515 protein binding
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length332 amino acids
FASTA sequence>sp|P00359|G3P3_YEAST Glyceraldehyde-3-phosphate dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH3 PE=1 SV=3 MVRVAINGFGRIGRLVMRIALSRPNVEVVALNDPFITNDYAAYMFKYDSTHGRYAGEVSHDDKHIIVDGKKIATYQERDPANLPWGSSNVDIAIDSTGVFKELDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEEKYTSDLKIVSNASCTTNCLAPLAKVINDAFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVKAAAEGKLKGVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA
Structure Information
PDB ID3PYM, 4IQ8
Quaternary structure
Function 1
Function descriptionGAPDH, glyceraldehyde 3-phosphate dehydrogenase
References for functionRingel AE, Ryznar R, Picariello H, Huang, KL, Lazarus, AG, Holmes SG. (2013) Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination. PLoS Genet. 9, e1003871
E.C. number1.2.1.12
Location of functional site(s)binding site: 33, 78, 120, 180, 232, 314; active site: 150; site: 177; nucleotide binding: 11-12
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds to Sir2, regulates gene silencing by influencing Sir2’s association with chromatin, especially near telomeres
References for functionRingel AE, Ryznar R, Picariello H, Huang, KL, Lazarus, AG, Holmes SG. (2013) Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination. PLoS Genet. 9, e1003871
E.C. number
Location of functional site(s)
Cellular location of functionnucleus
Commentsshowed mutant proteins deficient in catalytic activity can still function in other function