Protein Information

General Information
MoonProt ID353
First appeared in release2.0
Name(s)glycyl-tRNA synthetase, GlyRS, Glycine--tRNA ligase, Gene: GARS
UniProt IDP41250 (SYG_HUMAN)
GO termsGO:0006412 translation GO:0006418 tRNA aminoacylation for protein translation GO:0006426 glycyl-tRNA aminoacylation GO:0015966 diadenosine tetraphosphate biosynthetic process GO:0000166 nucleotide binding GO:0004081 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity GO:0004812 aminoacyl-tRNA ligase activity GO:0004820 glycine-tRNA ligase activity GO:0005524 ATP binding GO:0016787 hydrolase activity GO:0016874 ligase activity GO:0046983 protein dimerization activity GO:0005654 nucleoplasm GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0005759 mitochondrial matrix GO:0005829 cytosol GO:0030141 secretory granule GO:0030424 axon GO:0042995 cell projection GO:0070062 extracellular exosome GO:0005515 protein binding
Organisms for which functions have been demonstratedHomo sapiens (human)
Sequence length739 amino acids
FASTA sequence>sp|P41250|SYG_HUMAN Glycine--tRNA ligase OS=Homo sapiens GN=GARS PE=1 SV=3 MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
Structure Information
PDB ID2PME, 2Q5H, 2PMF, 4KQE, 2Q5I, 4KR2, 4KR3, 4QEI
Quaternary structure
Function 1
Function descriptionglycyl-tRNA synthetase, catalyze the formation of glycyl-tRNAGly as a substrate for ribosomal protein synthesis, P41250 (SYG_HUMAN), ATP + glycine + tRNA(Gly) => AMP + diphosphate + glycyl-tRNA(Gly) AND P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O => GTP + GMP
References for functionAntonellis, Anthony, et al. "Glycyl tRNA synthetase mutations in Charcot-Marie-Tooth disease type 2D and distal spinal muscular atrophy type V." The American Journal of Human Genetics 72.5 (2003): 1293-1299.
E.C. number3.6.1.17 AND 6.1.1.14 (two step process)
Location of functional site(s)binding site: 213, 299, 435; nucleotide binding: 331-333, 341-346, 457-458, 580-583
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds to NEDD8, E1, and E2 (Ubc12) in neddylation pathway, binds the APPBP1 subunit of E1 and captures and protects (like a chaperone) activated E2 (NEDD8-conjugated Ubc12) before the activated E2 reaches a downstream target
References for function27348078
E.C. number
Location of functional site(s)
Cellular location of functioncytoplasm
Comments