Protein Information

General Information
MoonProt ID361
First appeared in release2.0
Name(s)homocitrate synthase, Lys20, Homocitrate synthase, cytosolic isozyme Gene: LYS20
UniProt IDP48570 (HOSC_YEAST)
GO termsGO:0001207 histone displacement GO:0006281 DNA repair GO:0008652 cellular amino acid biosynthetic process GO:0009085 lysine biosynthetic process GO:0019752 carboxylic acid metabolic process GO:0019878 lysine biosynthetic process via aminoadipic acid GO:0003824 catalytic activity GO:0004410 homocitrate synthase activity GO:0005515 protein binding GO:0016740 transferase activity GO:0046912 transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer GO:0005634 nucleus GO:0005737 cytoplasm
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length428 amino acids
FASTA sequence>sp|P48570|HOSC_YEAST Homocitrate synthase, cytosolic isozyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS20 PE=1 SV=1 MTAAKPNPYAAKPGDYLSNVNNFQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGLKAKILTHIRCHMDDAKVAVETGVDGVDVVIGTSKFLRQYSHGKDMNYIAKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYELIRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGITPLGGLMARMIVAAPDYVKSKYKLHKIRDIENLVADAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKARVDQLNLNLTDDQIKEVTAKIKKLGDVRSLNIDDVDSIIKNFHAEVSTPQVLSAKKNKKNDSDVPELATIPAAKRTKPSA
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionhomocitrate synthase, enzyme, lysine biosynthetic pathway
References for functionRamos F., Verhasselt P., Feller A., Peeters P., Wach A., Dubois E., Volckaert G. Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae. Yeast 12:1315-1320(1996) PMID: 8923736
E.C. number2.3.3.14
Location of functional site(s)
Cellular location of function
Comments
Function 2
Function descriptionDNA damage repair, helps in INO80 accumulation at DNA breaks to aid in histone eviction
References for function25628362, 20810648
E.C. number
Location of functional site(s)C-terminal domain. Torres-Machorro AL, Aris JP, Pillus L. 2015. A moonlighting metabolic protein influences repair at DNA double-stranded breaks. Nucleic Acids Res 43:1646-1658. PMID: 25628362.
Cellular location of functionnucleus, chromatin
Commentsa mutation in catalytic activity can still function in DNA repair. Scott,E.M. and Pillus,L. (2010) Homocitrate synthase connects amino acid metabolism to chromatin functions through Esa1 and DNA damage. Genes Dev., 24, 1903–1913. PMID: 20810648