Protein Information

General Information
MoonProt ID374
First appeared in release2.0
Name(s)phosphofructokinase 1, PFK1,
UniProt IDP08237
GO termsGO:0005980 glycogen catabolic process GO:0006002 fructose 6-phosphate metabolic process GO:0006096 glycolytic process GO:0008152 metabolic process GO:0016310 phosphorylation GO:0032024 positive regulation of insulin secretion GO:0042593 glucose homeostasis GO:0046716 muscle cell cellular homeostasis GO:0046835 carbohydrate phosphorylation GO:0051259 protein oligomerization GO:0061615 glycolytic process through fructose-6-phosphate GO:0061621 canonical glycolysis GO:0093001 glycolysis from storage polysaccharide through glucose-1-phosphate GO:0000166 nucleotide binding GO:0003824 catalytic activity GO:0003872 6-phosphofructokinase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0008022 protein C-terminus binding GO:0008443 phosphofructokinase activity GO:0016301 kinase activity GO:0016740 transferase activity GO:0019900 kinase binding GO:0042802 identical protein binding GO:0042803 protein homodimerization activity GO:0046872 metal ion binding GO:0070061 fructose binding GO:0005737 cytoplasm GO:0005829 cytosol GO:0005945 6-phosphofructokinase complex GO:0016324 apical plasma membrane GO:0070062 extracellular exosome GO:0097228 sperm principal piece
Organisms for which functions have been demonstratedHomo sapiens (human)
Sequence length780 amino acids
FASTA sequence>sp|P08237|PFKAM_HUMAN ATP-dependent 6-phosphofructokinase, muscle type OS=Homo sapiens GN=PFKM PE=1 SV=2 MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV
Structure Information
PDB ID4OMT
Quaternary structure
Function 1
Function descriptionphosphofructokinase, enzyme in glycolysis, first committed step of glycolysis, by phosphorylating F6P into fructose-1,6-bisphosphate (F1,6P), ATP + D-fructose 6-phosphate => ADP + D-fructose 1,6-bisphosphate
References for functionYamasaki, Tomoyuki, et al. "Structure of the entire human muscle phosphofructokinase-encoding gene: a two-promoter system." Gene 104.2 (1991): 277-282.
E.C. number2.7.1.11
Location of functional site(s)binding site: 25, 201, 264, 292, 471, 566, 629, 655, 735; metal binding: 119; active site: 166; nucleotide binding: 88-89, 118-121
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds TEADs proteins and promotes their interaction with YAP/TAX transcription factors
References for functionEnzo E, Santinon G, Pocaterra A, Aragona M, Bresolin S, Forcato M, Grifoni D, Pession A, Zanconato F, Guzzo G, Bicciato S, Dupont S. Aerobic glycolysis tunes YAP/TAZ transcriptional activity. EMBO J. 2015 May 12;34(10):1349-70. doi: 10.15252/embj.201490379. PMID: 25796446
E.C. number
Location of functional site(s)
Cellular location of functionnucleus
Comments