Protein Information

General Information
MoonProt ID379
First appeared in release2.0
Name(s)pyruvate kinase 3 (PK3) isoform 2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1,THBP1, Tumor M2-PK, p58 Gene: PKM
UniProt IDP14618 (KPYM_HUMAN)
GO termsGO:0001666 response to hypoxia GO:0001889 liver development GO:0006006 glucose metabolic process GO:0006096 glycolytic process GO:0006754 ATP biosynthetic process GO:0007584 response to nutrient GO:0008152 metabolic process GO:0009629 response to gravity GO:0010033 response to organic substance GO:0012501 programmed cell death GO:0014870 response to muscle inactivity GO:0016310 phosphorylation GO:0031100 organ regeneration GO:0032868 response to insulin GO:0042866 pyruvate biosynthetic process GO:0043403 skeletal muscle tissue regeneration GO:0061621 canonical glycolysis GO:0000166 nucleotide binding GO:0000287 magnesium ion binding GO:0003824 catalytic activity GO:0004743 pyruvate kinase activity GO:0005515 protein binding GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0023026 MHC class II protein complex binding GO:0030955 potassium ion binding GO:0043531 ADP binding GO:0044822 poly(A) RNA binding GO:0046872 metal ion binding GO:0005634 nucleus GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0005829 cytosol GO:0005886 plasma membrane GO:0005929 cilium GO:0031012 extracellular matrix GO:0031982 vesicle GO:0043209 myelin sheath GO:0070062 extracellular exosome GO:1903561 extracellular vesicle GO:0005515 protein binding
Organisms for which functions have been demonstratedHomo sapiens (human)
Sequence length531 amino acids
FASTA sequence>sp|P14618|KPYM_HUMAN Pyruvate kinase PKM OS=Homo sapiens GN=PKM PE=1 SV=4 MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
Structure Information
PDB ID3GQY, 3SRD, 3U2Z, 4JPG, 1ZJH, 4G1N
Quaternary structure
Function 1
Function descriptionpyruvate kinase, ATP + pyruvate -> ADP + phosphoenolpyruvate, in glycolysis
References for functionKenzaburo, Tani, et al. "Human M 2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma." Gene 73.2 (1988): 509-516.
E.C. number2.7.1.40
Location of functional site(s)binding site: 70, 73, 106, 120, 207, 270, 295, 296, 328, 464, 482, 489; metal binding: 75, 77, 113, 114, 272, 296; site: 270, 433; nucleotide binding: 75-78
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptioninteractions with zona pellucida proteins of egg
References for functionPetit FM, Serres C, Bourgeon F, Pineau C, Auer J. (2013) Identification of sperm head proteins involved in zona pellucida binding. Hum. Reprod. 28, 852–865. PMID: 23355646.
E.C. number
Location of functional site(s)
Cellular location of functionsperm membrane
Comments