Protein Information

General Information
MoonProt ID380
First appeared in release2.0
Name(s)pyruvate decarboxylase, KIPdc1, Gene: KIPDC1
UniProt IDQ12629 (PDC1_KLULA)
GO termsGO:0008152 metabolic process GO:0000287 magnesium ion binding GO:0003824 catalytic activity GO:0004737 pyruvate decarboxylase activity GO:0016829 lyase activity GO:0016831 carboxy-lyase activity GO:0030976 thiamine pyrophosphate binding GO:0046872 metal ion binding GO:0006351 transcription, DNA-templated
Organisms for which functions have been demonstratedKluyveromyces lactis
Sequence length563 amino acids
FASTA sequence>sp|Q12629|PDC1_KLULA Pyruvate decarboxylase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN=PDC1 PE=1 SV=2 MSEITLGRYLFERLKQVEVQTIFGLPGDFNLSLLDNIYEVPGMRWAGNANELNAAYAADGYARLKGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVPSVSSQAKQLLLHHTLGNGDFTVFHRMSSNISETTAMITDINTAPAEIDRCIRTTYVSQRPVYLGLPANLVDLTVPASLLDTPIDLSLKPNDPEAEEEVIENVLQLIKEAKNPVILADACCSRHDAKAETKKLIDLTQFPAFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYSYKTKNIVEFHSDYTKIRSATFPGVQMKFALQKLLTKVADAAKGYKPVPVPSEPEHNEAVADSTPLKQEWVWTQVGEFLREGDVVITETGTSAFGINQTHFPNNTYGISQVLWGSIGFTTGATLGAAFAAEEIDPKKRVILFIGDGSLQLTVQEISTMIRWGLKPYLFVLNNDGYTIERLIHGETAQYNCIQNWQHLELLPTFGAKDYEAVRVSTTGEWNKLTTDEKFQDNTRIRLIEVMLPTMDAPSNLVKQAQLTAATNAKN
Structure Information
PDB ID2VJY
Quaternary structure
Function 1
Function descriptionpyruvate decarboxylase, 2-oxo acid -> an aldehyde + CO2
References for functionBianchi M.M., Tizzani L., Destruelle M., Frontall L., Wesolowski-Louvel M. The 'petite-negative' yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity. Mol. Microbiol. 19:27-36(1996). PMID: 8821934.
E.C. number4.1.1.1
Location of functional site(s)binding site: 28, 115, 477; metal binding: 444, 471, 473
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptiontranscriptional regulator but might not bind DNA directly (possibly by sequestering Rag3 in the cytoplasm), inhibits transcription from PDC1 and PDC5 promoters
References for function24763423, 10219994
E.C. number
Location of functional site(s)
Cellular location of functioncytoplasm
Commentstranscriptional regulation does not require catalytic activity shown by studies with mutant protein