Protein Information

General Information
MoonProt ID392
First appeared in release2.0
Name(s)threonyl aminoacyl-tRNA synthetase, TARS, Threonine-tRNA ligase, cytoplasmic, Gene:TARS
UniProt IDA8KC60 (A8KC60_DANRE)
GO termsGO:0001944 vasculature development GO:0006418 tRNA aminoacylation for protein translation GO:0006435 threonyl-tRNA aminoacylation GO:0043039 tRNA aminoacylation GO:0000166 nucleotide binding GO:0004812 aminoacyl-tRNA ligase activity GO:0004829 threonine-tRNA ligase activity GO:0005524 ATP binding GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds GO:0005737 cytoplasm GO:0001944 vasculature development
Organisms for which functions have been demonstratedDanio rerio (Zebrafish) (Brachydanio rerio)
Sequence length718 amino acids
FASTA sequence>tr|A8KC60|A8KC60_DANRE Zgc:92586 protein OS=Danio rerio GN=tars PE=2 SV=1 MADESVTDQLKELKMEEKKNTKDGGKKKNKNAAGDSGGKTELSPPPQYIDERLSIYNKLKEEHDALLAEKAAKESKPIKITLPDGKVVDGESWKTTPYQVACGISQGLADNTVISKVNNGVWDLDRPLEEDCSLVFLKFDDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLDNEGVSSNDFPCLENLCKKIIKEKQPFERLEVKKETLLEMFKYNKFKCRILNEKVTTPTTTVYRCGPLIDLCRGPHVRHTGKIKAMKIHKNSSTYWEGKADMESLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKLGREQDLFFFHDLSPGSCFFLPKGAYIYNTLIEFIRSEYRKRGFQEVVTPNIYNSKLWQTSGHWQHYSENMFSFEVEKEIFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCSMDQIESEIKGCLDFLRTVYDVFGFTFKLNLSTRPEKFLGEPEVWDQAEKQLENSLNDFGEKWVLNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTFVSHDGDDKKRPVIIHRAILGSVERMIAILTENYGGKWPLWLSPRQVMVVPIGPTCDEYAQRVQKEFHKVGLMTDVDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKSSDTVNVRTRDNKVHGERSLTDCMERLKQLKASRTRNAEEEF
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionthreonyl aminoacyl-tRNA synthetase, attaches threonine to tRNA, early step in protein synthesis, ATP + L-threonine + tRNA(Thr) -> AMP + diphosphate + L-threonyl-tRNA(Thr)
References for functionCao, Zigang, et al. "Noncanonical function of threonyl-tRNA synthetase regulates vascular development in zebrafish." Biochemical and biophysical research communications 473.1 (2016): 67-72.
E.C. number6.1.1.3
Location of functional site(s)
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionpromotes vascular development, demostrated this is separate from role in protein synthesis
References for function26271225
E.C. number
Location of functional site(s)
Cellular location of functionextracellular
Commentssecreted from endothelial cells in response to TNF-? and VEGF, association between TARS expression and advancing stage of ovarian cancer, mutation that prevents synthetase activity is still active in promoting vascular development, single amino acid substitutions in TARS can block the anti-angiogenic effects of BC194, indicating that TARS is the specific target of BC194