Protein Information

General Information
MoonProt ID65
First appeared in release1.0
Name(s)alpha subunit of 6-phosphofructokinase Phosphofructokinase Phosphohexokinase Gene Name:PFK1
UniProt IDQ92448 (K6PF1_PICPA), Reviewed
GO termsGO:0006002 fructose 6-phosphate metabolic process GO:0006096 glycolytic process GO:0008152 metabolic process GO:0016310 phosphorylation GO:0046835 carbohydrate phosphorylation GO:0000166 nucleotide binding GO:0003824 catalytic activity GO:0003872 6-phosphofructokinase activity GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0046872 metal ion binding GO:0005737 cytoplasm GO:0005945 6-phosphofructokinase complex GO:0030242 autophagy of peroxisome
Organisms for which functions have been demonstratedKomagataella pastoris (Yeast) (Pichia pastoris)
Sequence length989
FASTA sequence>gi|254568386|ref|XP_002491303.1| Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis [Komagataella pastoris GS115] MPEPSISALSFTSFVTNDDKLFEETFNFYTKLGFHATRSYVKDNRSDFELTGISTDSIKEIWLESFPLSEVVETSAGRELRKPLQESVGYQSEALLGYSPYQSDGVVIKLRLSNHDLQKNKDLPGEVTFFTASIDKLRAKLIEIGAEIIPSEIDLVEFSTKDPMGDVISFSSYPSLSSKKITSPDFFLHPKKEVRSQESIVEQVKSEEGKKKIAIITSGGDAPGMNAAVRAVTRAGIFYGCKVYACYEGYTGLVKGGDMLKELQWQDVRGLLSIGGTIIGTARSKEFRERWGRLQACYNMVSNGIDALVVCGGDGSLTGADLFRNEWPELIKELLGEGKITKEQYETHRNLTIVGLVGSIDNDMCGTDSTIGAYSSLERIIELVDYIDATAASHSRAFVVEVMGRHCGWLGLMSGIATGADYIFIPERPPSETNWKDDLKKVCLRHREKGRRKTTVIVAEGAIDDQLNPITSEEVKDVLVEIGLDTRITRLGHVQRGGAPCAFDRFLATVQGVDAVRAVLESTPAIPSPVISILENKIVRQPLVESVAQTKTVSDAIEAKDFDKALKLRDQEFATSYESFLSVSKYDDGSYLVPESSRLNIAIIHVGAPTSALNPATRVATLNSLAKGHRVFAIRNGFAGLIRHGAVRELNWIDVEDWHNTGGSEIGTNRSLPSDDMGTVAYYFQQYKFDGLIIIGGFEAFTALYQLDAARAQYPIFNIPMCCLPATVSNNVPGTEYSLGSDTCLNTLSGYCDAVKQSASASRRRTFVVEVQGGYSGYLASYAGLITGALAVYTPENPINLQTVQEDIELLTRTYEEDDGKNRSGKIFIHNEKASKVYTTDLIAAIIGEAGKGRFESRTAVPGHVQQGKSPSSIDRVNACRLAIKCCNFIEDANFQVKHNANLSADERHLRFFYDDGVKTSAVSGKSSVIDDNTSVVIGIQGSEVTFTPVKQLWEKETHHKWRKGKNVHWEQLNIVSDLLSGRLSIRTT
Structure Information
PDB ID3OPY
Quaternary structure
Function 1
Function descriptionphosphofructokinase, enzyme ATP + D-fructose 6-phosphate => ADP + D-fructose 1,6-bisphosphate Glycolysis
References for function
E.C. number2.7.1.11
Location of functional site(s)
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionprotein, but not catalytic function, is required for microautophagy (vacuolar degradation of peroxisomes) only alpha subunit of phosphofructokinase plays this role
References for functionYuan W, Tuttle DL, Shi YJ, Ralph GS, Dunn WA. Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase. J Cell Sci. 1997 Aug. PMID: 9296392
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm
Comments