Protein Information

General Information
MoonProt ID76
First appeared in release1.0
Name(s)Enolase 2 2-phospho-D-glycerate hydro-lyase 2 2-phosphoglycerate dehydratase 2 Gene Name:ENO2
UniProt IDP00925 (ENO2_YEAST), Reviewed
GO termsGO:0006094 gluconeogenesis GO:0006096 glycolytic process GO:0032889 regulation of vacuole fusion, non-autophagic GO:0000287 magnesium ion binding GO:0004634 phosphopyruvate hydratase activity GO:0005515 protein binding GO:0016829 lyase activity GO:0046872 metal ion binding GO:0000015 phosphopyruvate hydratase complex GO:0000324 fungal-type vacuole GO:0005737 cytoplasm GO:0005739 mitochondrion GO:0009898 cytoplasmic side of plasma membrane GO:0005773 vacuole GO:0061025 membrane fusion
Organisms for which functions have been demonstratedSaccharomyces cerevisiae
Sequence length437
FASTA sequence>sp|P00925|ENO2_YEAST Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2 MAVSKVYARSVYDSRGNPTVEVELTTEKGVFRSIVPSGASTGVHEALEMRDEDKSKWMGKGVMNAVNNVNNVIAAAFVKANLDVKDQKAVDDFLLSLDGTANKSKLGANAILGVSMAAARAAAAEKNVPLYQHLADLSKSKTSPYVLPVPFLNVLNGGSHAGGALALQEFMIAPTGAKTFAEAMRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAEEALDLIVDAIKAAGHDGKVKIGLDCASSEFFKDGKYDLDFKNPESDKSKWLTGVELADMYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAGIQIVADDLTVTNPARIATAIEKKAADALLLKVNQIGTLSESIKAAQDSFAANWGVMVSHRSGETEDTFIADLVVGLRTGQIKTGAPARSERLAKLNQLLRIEEELGDKAVYAGENFHHGDKL
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionenolase, enzyme 2-phospho-D-glycerate <=> phosphoenolpyruvate + H2O Glycolysis
References for function
E.C. number4.2.1.11
Location of functional site(s)
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionrequired for vacuole homotypic membrane fusion and protein trafficking to the vacuole enzyme catalytic activity not needed for this role
References for functionDecker BL, Wickner WT. Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast. J Biol Chem. 2006 May 19. PMID: 16565073
E.C. numberN/A
Location of functional site(s)
Cellular location of functionVacuole, surface of vacuole
Comments
Third Function
Function descriptionbinds to tRNA-Lys-CUU (tRK2) in cytoplasm and delivers it to mitochondria for import into the mitochondria
References for functionEntelis N, Brandina I, Kamenski P, Krasheninnikov IA, Martin RP, Tarassov I. A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae. Genes Dev. 2006 Jun 15. PMID: 16738406
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncytoplasm, cytoplasmic side of mitochondrial membrane
Comments