Protein Information

General Information
MoonProt ID81
First appeared in release1.0
Name(s)Malate synthase Gene Name: glcB
UniProt IDP9WK17
GO termsGO:0006097 glyoxylate cycle GO:0006099 tricarboxylic acid cycle GO:0003824 catalytic activity GO:0004474 malate synthase activity GO:0016740 transferase activity GO:0046872 metal ion binding GO:0005737 cytoplasm GO:0005515 protein binding GO:0098631 cell adhesion mediator
Organisms for which functions have been demonstratedMycobacterium tuberculosis
Sequence length741 aa
FASTA sequence>sp|P9WK17|MASZ_MYCTU Malate synthase G OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=glcB PE=1 SV=1 MTDRVSVGNLRIARVLYDFVNNEALPGTDIDPDSFWAGVDKVVADLTPQNQALLNARDELQAQIDKWHRRRVIEPIDMDAYRQFLTEIGYLLPEPDDFTITTSGVDAEITTTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDVIPETDGAEKGPTYNKVRGDKVIAYARKFLDDSVPLSSGSFGDATGFTVQDGQLVVALPDKSTGLANPGQFAGYTGAAESPTSVLLINHGLHIEILIDPESQVGTTDRAGVKDVILESAITTIMDFEDSVAAVDAADKVLGYRNWLGLNKGDLAAAVDKDGTAFLRVLNRDRNYTAPGGGQFTLPGRSLMFVRNVGHLMTNDAIVDTDGSEVFEGIMDALFTGLIAIHGLKASDVNGPLINSRTGSIYIVKPKMHGPAEVAFTCELFSRVEDVLGLPQNTMKIGIMDEERRTTVNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAGPMVRKGTMKSQPWILAYEDHNVDAGLAAGFSGRAQVGKGMWTMTELMADMVETKIAQPRAGASTAWVPSPTAATLHALHYHQVDVAAVQQGLAGKRRATIEQLLTIPLAKELAWAPDEIREEVDNNCQSILGYVVRWVDQGVGCSKVPDIHDVALMEDRATLRISSQLLANWLRHGVITSADVRASLERMAPLVDRQNAGDVAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILHRRRREFKARAAEKPAPSDRAGDDAAR
Structure Information
PDB ID1N8I 3S9I 3S9Z 3SAD 3SAZ 3SB0 2GQ3
Quaternary structure
Function 1
Function descriptionMalate synthase, enzyme Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA Acetyl-CoA + H2O + glyoxylate => malate + CoA Carbohydrate metabolism; glyoxylate cycle glyoxylate pathway enables bacteria to assimilate two-carbon compounds into the TCA cycle
References for functionManabe, Yukari C., and William R. Bishai. "Latent Mycobacterium tuberculosis-persistence, patience, and winning by waiting." Nature medicine 6.12 (2000): 1327.
E.C. number2.3.3.9
Location of functional site(s)Active site:339,633 Binding site:434,305,543,633,275,118,312,339 Metal binding site:434,462
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinds fibronectin and laminin and A549 lung epithelial cells
References for functionKinhikar AG, Vargas D, Li H, Mahaffey SB, Hinds L, Belisle JT, Laal S. Mycobacterium tuberculosis malate synthase is a laminin-binding adhesin. Mol Microbiol. 2006 May;60(4):999-1013. PMID: 16677310
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncell surface
Comments