Protein Information

General Information
MoonProt ID93
First appeared in release1.0
Name(s)Pyruvate kinase PK Gene Name: pyk
UniProt IDQ07637 (KPYK_LACLA), Reviewed
GO termsGO:0006096 glycolysis GO:0008152 metabolic process GO:0016310 phosphorylation GO:0000166 nucleotide binding GO:0000287 magnesium ion binding GO:0003824 catalytic activity GO:0004743 pyruvate kinase activity GO:0005524 ATP binding GO:0016301 kinase activity GO:0016740 transferase activity GO:0030955 potassium ion binding GO:0046872 metal ion binding GO:0005515 protein binding GO:0098631 cell adhesion mediator
Organisms for which functions have been demonstratedLactococcus lactis IL1403
Sequence length502
FASTA sequence>sp|Q07637|KPYK_LACLA Pyruvate kinase OS=Lactococcus lactis subsp. lactis (strain IL1403) GN=pyk PE=3 SV=2 MNKRVKIVSTLGPAVEIRGGKKFGESGYWGESLDVEASAKNIAALIEEGANVFRFNFSHGDHPEQGARMATVHRAEEIAGHKVGFLLDTKGPEMRTELFADGADAISVVTGDKFRVATKQGLKSTPELIALNVAGGLDIFDDVEIGQTILIDDGKLGLSLTGKDAATREFEVEAQNDGVIGKQKGVNIPNTKIPFPALAERDDADIRFGLSQPGGINFIAISFVRTANDVKEVRRICEETGNPHVQLLAKIENQQGIENLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKLIISKVNKAGKIVVTATNMLESMTYNPRATRSEISDVFNAVIDGTDATMLSGESANGKYPRESVRTMATVNKNAQTMLKEYGRLHPERYDKSTVTEVVAASVKNAAEAMDIKLIVALTESGNTARLISKHRPNADILAITFDEKVERGLMINWGVIPTMTEKPSSTDDMFEVAEKVALASGLVESGDNIIIVAGVPVGTGRTNTMRIRTVK
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionPyruvate kinase, enzyme ADP + phosphoenolpyruvate => ATP + pyruvate Carbohydrate degradation, glycolysis
References for functionLlanos, R. M., et al. "Identification of a novel operon in Lactococcus lactis encoding three enzymes for lactic acid synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase." Journal of bacteriology 175.9 (1993): 2541-2551.
E.C. number2.7.1.40
Location of functional site(s)Binding site:308,275,54,276 Metal binding site:252,89,58,88,276,56 Other functional site:250
Cellular location of functioncytoplasm
Comments
Function 2
Function descriptionbinding to invertase, a hyperglycosylated mannoprotein from Saccharomyces cerevisiae
References for functionKatakura Y, Sano R, Hashimoto T, Ninomiya K, Shioya S. 2010. Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan. Appl Microbiol Biotechnol. 2010 Mar;86(1):319-26. doi: 10.1007/s00253-009-2295-y. Epub 2009 Nov 7. PMID: 19898842
E.C. numberN/A
Location of functional site(s)
Cellular location of functioncell surface
Comments